儿童空气中苯并(a)芘暴露作为基因测定细胞死亡的修饰因子

Q4 Medicine
O. Dolgikh, N. Nikonoshina
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引用次数: 0

摘要

介绍在空气源性暴露于苯并(a)芘的条件下,研究儿童的基因确定的细胞死亡特征与鉴定技术源性化学因子暴露的免疫和遗传标志物有关。材料和方法。五百六十九名学龄前儿童接受了检查。观察组包括384名生活在苯并(a)芘空气暴露条件下的儿童。对照组由185名生活在相对干净地区的儿童组成。采用高效液相色谱法测定了大气和血液中苯并(a)芘的含量。流式细胞荧光法测定膜联蛋白-FITC+7AAD-、膜联蛋白-FITC+7AAD+、Bax、Bcl-2、CD95+-、p53、TNFR。采用实时PCR方法对FAS(rs1159120)和TP53(rs1042522)基因多态性进行研究。后果与对照组和参考水平相比,0.000163 mg/(kg·day)剂量的产气苯并(a)芘暴露(7.4 MPCad)导致儿童血液中的污染水平增加(p<0.05)(在代偿性抗凋亡蛋白Bcl-2过量产生的背景下,凋亡标记物——膜联蛋白-FITC+7AAD细胞、CD3+CD95+淋巴细胞、p53、TNFR的含量增加)与C等位基因相关(OR=1.38;95%CI:1.02–1.88,p<0.05);FAS基因rs1159120的CC基因型(OR=2.53;95%CI:1.72–3.72,p<0.05),t TP53基因rs1042522的C等位基因(OR=1.96;95%CI:1.53–2.53,p<0.05)和CC基因型。局限性对使用所选方法的可能性和研究对象的特征进行研究没有任何限制。结论与苯并(a)芘(AnnexinV FITC+7AAD和CD3+CD95+淋巴细胞、p53、TNFR、Bcl-2细胞过量)血液污染相关的免疫谱变化与C等位基因相关(OR=1.38;95%CI:1.02–1.88,p<0.05);FAS基因(rs1159120)的CC基因型(OR=2.53;95%CI:1.72–3.72,p<0.05)、t TP53基因(rs1042522)的C等位基因(OR=1.96;95%CI:1.53–2.53,p<0.05)和CC基因型,当它以超过0.000163mg/(kg·day)的剂量进入人体时。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Aerogenic exposure of benzo(a)pyrene in children as the modification factor of genetically determined cell death
Introduction. The study of genetically determined cell death features in children under the conditions of aerogenic exposure to benzo(a)pyrene is relevant in the identification of immunological and genetic markers of technogenic chemical factor exposure. Materials and methods. Five hundred sixty nine preschool children were examined. Observation group included 384 children living under the conditions of aerogenic exposure to benzo(a)pyrene. Comparison group consisted of 185 children living in a relatively clean area. Determination of the content of benzo(a)pyrene in atmospheric air and in blood was carried out by HPLC. Determination of Annexin-FITC+7AAD–, Annexin-FITC+7AAD+, Bax, Bcl-2, CD95+-, p53, TNFR was made by flow cytofluorometry. The study of FAS (rs1159120) and TP53 (rs1042522) gene polymorphism was performed by real-time PCR. Results. The aerogenic benzo(a)pyrene exposure (7.4 MPCad) at a dose of 0.000163 mg/(kg · day) causes an increase in the level of contamination in children blood relative to the comparison group and the reference level (p<0.05). Changes in the immune profile of the examined contingent (increased content of apoptosis markers – Annexin-FITC+7AAD–-cells, CD3+CD95+-lymphocytes, p53, TNFR against the background of compensatory anti-apoptotic protein Bcl-2 hyperproduction) are associated with the C-allele (OR=1.38; 95% CI: 1.02–1.88, p<0.05); and CC-genotype (OR=2.53; 95% CI: 1.72–3.72, p<0.05) of FAS gene (rs1159120), and the C-allele (OR=1.96; 95% CI: 1.53–2.53, p<0.05) and CC-genotype (OR=2.53; 95% CI: 1.72–3.72, p<0.05) of t TP53 gene (rs1042522). Limitations. There are no restrictions on conducting research related to the possibility of using the selected methods and the characteristics of the objects of research. Conclusion. Changes in the immune profile associated with blood contamination with benzo(a)pyrene (excess of AnnexinV-FITC+7AAD– and CD3+CD95+-lymphocytes, p53, TNFR, Bcl-2 cells) are associated with the C-allele (OR=1.38; 95% CI: 1.02–1.88, p<0.05); and CC-genotype (OR=2.53; 95% CI: 1.72–3.72, p<0.05) of FAS gene (rs1159120), and C-allele (OR=1.96; 95% CI: 1.53–2.53, p<0.05) and CC-genotype (OR=2.53; 95% CI: 1.72–3.72, p<0.05) of t TP53 gene (rs1042522) form the risks of programmed cell death violations in children living under the conditions of aerogenic exposure to benzo(a)pyrene, when it is entered the body at a dose of more than 0.000163 mg/(kg · day).
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来源期刊
Gigiena i sanitariia
Gigiena i sanitariia Environmental Science-Pollution
CiteScore
0.80
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0.00%
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192
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