抗氧化疗法（维生素C）在实验诱导的卵巢子宫内膜异位症小鼠模型中的有效性

IF 2.7 Q2 OBSTETRICS & GYNECOLOGY

Womens Health Pub Date : 2022-01-01 DOI:10.1177/17455057221096218

Hayedeh Hoorsan, M. Simbar, F. Tehrani, F. Fathi, N. Mosaffa, H. Riazi, L. Akradi, Sherko Nasseri, Shayan Bazrafkan

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引用次数: 5

摘要

目的：本研究探讨维生素C对小鼠子宫内膜异位症卵巢诱导模型中子宫内膜病变和繁殖力障碍的治疗作用。方法：14只NMRI雌性小鼠（治疗组，N = 7）和（对照组，N = 7）。第二次手术（评估子宫内膜异位植入物）三天后，将小鼠随机分为两个干预组：对照组（安慰剂）和治疗组（每两天口服50 mg/kg维生素C，持续四周）。在发情期，处死小鼠。在宏观评估中，对子宫内膜异位植入物的大小、体积、重量、生长评分和粘附评分进行评估。显微镜评估检查了卵巢组织（窦卵泡、黄体和闭锁卵泡的数量）和子宫内膜异位病变（组织学和三色纤维化评分）。结果：与对照组（安慰剂）相比，治疗组（维生素C）治疗后植入物体积、生长评分、粘附程度评分和粘附严重程度评分显著降低（p < 0.0001）。两组（治疗组和对照组）子宫内膜异位植入物的中位重量、植入物组织的上皮化、三色纤维化评分和卵泡数之间的差异具有统计学意义（p < 维生素C治疗后萎缩卵泡明显减少（p < 0.05）。尽管对照组的标本中黄体数量似乎保存得更多，但两组的组织学评分之间没有统计学意义。结论：因此，我们可能暗示维生素C对减少子宫内膜植入物的诱导和生长，改善卵巢的生殖功能，从而预防子宫内膜异位症相关癌症具有显著作用。需要进一步的研究来改进有针对性的干预措施，从而预防和治疗人类子宫内膜异位症。

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The effectiveness of antioxidant therapy (vitamin C) in an experimentally induced mouse model of ovarian endometriosis

Objectives: This study investigates the therapeutic effect of vitamin C on the development of endometrial lesions and fecundity disorders in the ovarian induction model of mouse endometriosis. Methods: Ovarian endometriosis was surgically induced in 14 NMRI female mice (treatment group, N = 7) and (control group, N = 7). Three days after the second surgery (to assess endometriotic implant), the mice were randomized into two intervention groups: control (placebo) and treatment (50 mg/kg vitamin C every two days orally for four weeks) groups. In the oestrus phase, the mice were sacrificed. In macroscopic assessment, endometriotic implants were evaluated in size, volume, weight, growth score and adhesion score. The microscopic assessment examined the ovarian tissue (the number of antral follicles, corpus luteum and atretic follicles) and endometriotic lesion (histologic and trichrome fibrosis scores). Results: Post-treatment implant volume, growth score, adhesion extent score and adhesion severity score were significantly lower in the treatment group (vitamin C) in comparison with the control group (placebo) (p < 0.0001). The difference between the median weight of endometriotic implants, epithelialization of implant tissue, trichrome fibrosis scores and follicle number in the two groups (treatment and control) was statistically significant (p < 0.05). Atretic follicles were significantly decreased after vitamin C therapy (p < 0.05). Although the numbers of corpus luteum seemed to be more preserved in specimens from the control group, there was no statistical significance between the two groups’ histological scores. Conclusion: As a result, we may imply that vitamin C has a significant effect on reducing the induction and growth of endometrial implants, improving the fecundity function of ovaries, and consequently prevention of endometriosis-associated cancers. Further research is needed to improve targeted interventions resulting in the prevention and treatment of human endometriosis.

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来源期刊

Womens Health OBSTETRICS & GYNECOLOGY-

CiteScore

2.80

自引率

4.20%

发文量

审稿时长

15 weeks

期刊介绍： For many diseases, women’s physiology and life-cycle hormonal changes demand important consideration when determining healthcare management options. Age- and gender-related factors can directly affect treatment outcomes, and differences between the clinical management of, say, an adolescent female and that in a pre- or postmenopausal patient may be either subtle or profound. At the same time, there are certain conditions that are far more prevalent in women than men, and these may require special attention. Furthermore, in an increasingly aged population in which women demonstrate a greater life-expectancy.