家禽羽毛二硫键断裂,实现生物基聚合物生产

Q2 Materials Science
T. McGauran, N. Dunne, B. Smyth, E. Cunningham, M. Harris
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引用次数: 4

摘要

随着塑料生产所需的石油供应急剧减少,可持续资源中的替代聚合物有了明显的驱动力。家禽羽毛含有~90%的角蛋白,是天然聚合物的来源之一,具有巨大的生物聚合物生产潜力。然而,被称为二硫键的交联的存在阻碍了加工性。本文综述了通过使用还原剂(亚硫酸钠和硫酸钠)和水解来破坏二硫键的技术。使用FTIR和DSC分析样品,以量化可实现的键断裂、对热性能的影响和蛋白质浓度的变化。还对颗粒尺寸对二硫化物键断裂的影响进行了综述,并对键后处理的改造进行了量化。最后,使用二辛可宁酸(BCA)蛋白质测定法来量化可溶性蛋白质含量的变化,这是预测是否会形成生物聚合物的关键。结果表明,使用这些技术可以实现48%和67%之间的最终二硫键断裂。还表明,二硫化物键含量在处理后表现出高达60%的键重组。二硫键的这些减少增加了热塑性性质和表观蛋白质含量。尽管达到了最高的键断裂百分比,但水解导致有用蛋白质的降解,使该材料不适合生产生物聚合物。结果表明,用亚硫酸钠(4.3重量%的羽毛)处理和使用小颗粒(0–100µm)充分改变了生羽毛的性质,从而能够生产羽毛生物聚合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Poultry feather disulphide bond breakdown to enable bio-based polymer production
With oil supplies, needed for plastic production, decreasing dramatically, there is a clear driver for alterative polymers from sustainable resources. Poultry feathers, containing ∼90% keratin, are one source of natural polymer with huge potential for biopolymer production. However, the presence of crosslinks, known as disulphide bonds, hinders processability. This paper reviews techniques to enable breakage of disulphide bonds through use of reduction agents (sodium sulphite and sodium sulphate) and hydrolysis. Samples were analysed using FTIR and DSC to quantify achievable bond breakage, effect on thermal properties and changes in protein concentration. A review on the effect of particle size on disulphide bond breakage was also conducted, along with quantifying the reformation of bonds post-processing. Finally, a bicinchoninic acid (BCA) protein assay was used to quantify changes to soluble protein content, key to predicting if biopolymer formation can occur. The results showed a final disulphide bond breakage of between 48% and 67% was achievable using these techniques. It was also shown that disulphide bond content exhibited up to 60% bond reformation post treatment. These reductions in disulphide bonds increased the thermoplastic nature and apparent protein content. Despite achieving the highest bond breakage percentage, hydrolysis caused degradation of useful proteins, rendering the material unsuitable for biopolymer production. Results suggested that treatment with sodium sulphite (4.3% wt. of feathers) and use of a small particle size (0–100 µm), sufficiently altered the properties of raw feathers to enable feather biopolymer production.
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来源期刊
Polymers from Renewable Resources
Polymers from Renewable Resources Materials Science-Polymers and Plastics
CiteScore
3.50
自引率
0.00%
发文量
15
期刊介绍: Polymers from Renewable Resources, launched in 2010, publishes leading peer reviewed research that is focused on the development of renewable polymers and their application in the production of industrial, consumer, and medical products. The progressive decline of fossil resources, together with the ongoing increases in oil prices, has initiated an increase in the search for alternatives based on renewable resources for the production of energy. The prevalence of petroleum and carbon based chemistry for the production of organic chemical goods has generated a variety of initiatives aimed at replacing fossil sources with renewable counterparts. In particular, major efforts are being conducted in polymer science and technology to prepare macromolecular materials based on renewable resources. Also gaining momentum is the utilisation of vegetable biomass either by the separation of its components and their development or after suitable chemical modification. This journal is a valuable addition to academic, research and industrial libraries, research institutions dealing with the use of natural resources and materials science and industrial laboratories concerned with polymer science.
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