戊型肝炎病毒监测结果及其实验室筛查算法

T. Amvrosieva, N. Paklonskaya, Y. Kaltunova, I. Belskaya, E. Kishkurno
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引用次数: 0

摘要

背景白俄罗斯共和国每年都会记录个别戊型病毒性肝炎病例,这表明需要在人口和水库两个层面进行病原体监测。客观的整合2018-2021年戊型肝炎病毒监测数据,并制定有效的实验室筛查算法。材料和方法。作为研究的一部分,对345个样本进行了分析,其中包括227个人体生物样本、37个家猪生物材料样本、22个食物样本和59个废水样本。后果根据血清诊断结果,肾移植组(n=29)对戊型肝炎病毒IgM和IgG的检出率分别为6.9%[0.85%;23.03%]和17.2%[7.13%;35.02%];妊娠病理组(n=44)分别为6.8%[1.68%;18.89%和11.4%[4.5%;24.43%。在病因不明的急性肝炎患者(n=26)中,未检测到抗病毒IgM,而抗病毒IgG检测频率达到7.7%[0.02%;25.26]。在对照组(献血者,n=53)中,IgM和IgG分别检测到1.9%[0.6%;10.88%]和5.7%[1.35%;15.97]。在来自肾脏接受者的8个人体生物样本(3.8%)中检测到戊型肝炎病毒RNA。已鉴定的戊型肝炎病毒以基因型GIII为代表,属于以前未鉴定的亚基因型(GIIIa-GIIIi)。在所研究的猪生物材料样本以及食品和废水样本中,没有检测到戊型肝炎病毒RNA。结论。已经开发并测试了一种用于戊型肝炎病毒实验室筛查的算法。其关于戊型病毒性肝炎诊断的章节载于《戊型病毒型肝炎实验室诊断算法使用说明书》(2021年1月28日第148-1220号)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
HEPATITIS E VIRUS MONITORING RESULTS AND ITS LABORATORY SCREENING ALGORITHM
Background. Individual cases of viral hepatitis E are recorded in the Republic of Belarus annually indicating the need for the pathogen monitoring at both the population and reservoir levels. Objective. To consolidate the monitoring data on hepatitis E virus over the period of 2018 - 2021, as well as to work out an effective algorithm for its laboratory screening. Material and methods. As part of the study, 345 samples were analyzed, including 227 human biological samples, 37 samples of biological materials of domestic pigs, 22 samples of food and 59 samples of waste water. Results. According to the results of serum diagnostics, in the group of kidney recipients (n = 29), the detection rate of IgM and IgG to hepatitis E virus was 6.9% [0.85%; 23.03%] and 17.2% [7.13%; 35, 02%] respectively; in the group of patients with pregnancy pathology (n = 44) - 6.8% [1.68%; 18.89%] and 11.4% [4.5%; 24.43%] respectively. In patients with acute hepatitis of unknown etiology (n = 26), antiviral IgM was not detected, while the frequency of antiviral IgG detection reached 7.7% [1.02%; 25.26]. In control group (blood donors, n = 53) IgM and IgG were detected in 1.9% [0.6%; 10.88%] and 5.7% [1.35%; 15.97] of those examined respectively. Hepatitis E virus RNA was detected in 8 human biological samples (3.8%) from kidney recipients. The identified hepatitis E viruses were represented by genotype GIII and belonged to a previously unidentified subgenotype (GIIIa - GIIIi). In the studied samples of biological material from pigs, as well as in samples of food and waste water, hepatitis E virus RNA was not detected. Conclusions. An algorithm for hepatitis E virus laboratory screening has been developed and tested. Its section concerning the diagnosis of viral hepatitis E is set out in the Instructions for use "Algorithm for laboratory diagnosis of viral hepatitis E" (No. 148-1220 from January 28, 2021).
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