尼日利亚获得的大肠杆菌抗微生物基因的计算机基因组分析

Q3 Agricultural and Biological Sciences

Journal of Food Quality and Hazards Control Pub Date : 2022-01-05 DOI:10.18502/jfqhc.8.4.8260

O. Nwaiwu, Helen Onyeaka

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引用次数: 0

摘要

背景：抗微生物耐药性是一个全球性问题，对公共卫生和经济产生巨大影响。本研究旨在了解从尼日利亚不同食物来源和环境中分离的大肠杆菌基因组中获得的抗微生物基因序列。方法：采用KmerResistance 2.2软件对272株分离株的基因组组装进行计算机分析，以确定获得性耐药基因的流行率。结果：共检测到107个耐药基因，其中包括编码24种超广谱β-内酰胺酶的基因。在分析的90%的基因组中发现了潜在的多药耐药性。所分析的所有菌株都含有至少一个抗性基因序列，并且具有高度相似性或同源性（95%ID及以上）。两株菌株携带超过30个抗微生物基因序列，在24株菌株中检测到超过20个基因。结论：在所有分析的基因组中发现的抗性基因都是种内获得的，而不是种间获得的。这为进一步研究检测到的基因的同源性提供了机会，所获得的数据有助于监测食品和环境中大肠杆菌抗性基因的流行病学。

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Acquired Antimicrobial Resistance Genes of Escherichia coli Obtained from Nigeria: In silico Genome Analysis

Background: Antimicrobial resistance is a global problem with enormous public health and economic impact. This study was carried out to get an overview of acquired antimicrobial resistance gene sequences in the genomes of E. coli isolated from different food sources and the environment in Nigeria. Methods: To determine the acquired antimicrobial-resistant genes prevalence, genome assemblies of 272 isolates were analyzed In silico with KmerResistance 2.2 software. Results: A total of 107 antimicrobial resistance genes, which included genes that encode for 24 extended-spectrum beta-lactamases were detected. Potential multidrug resistance was found in 90% of the genomes analyzed. All strains analyzed contained at least one resistant gene sequence and had high similarity or homology (95% ID and above). Two strains harboured over 30 sequences of antimicrobial resistant genes, and in 24 strains over 20 genes were detected. Conclusion: The resistant genes found in all the genomes analyzed were acquired intra-species and not inter-species. This provides an opportunity for further studies of the orthologous nature of the genes detected and the data obtained can help monitor the epidemiology of E. coli resistant genes in the food and environment.

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来源期刊

Journal of Food Quality and Hazards Control Agricultural and Biological Sciences-Food Science

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Journal of Food Quality and Hazards Control (J. Food Qual. Hazards Control) is an international peer-reviewed quarterly journal that aims at publishing of high quality articles involved in food quality, food hygiene, food safety, and food control which scientists from all over the world may submit their manuscript. This academic journal aims to improve international exchange of new findings and recent developments in all aspects of agricultural and biological sciences. This free of charge journal is published in both online and print forms and welcomes the manuscripts that fulfill the general criteria of novelty and scientific importance. Among the most significant objectives of Journal of Food Quality and Hazards Control are to ensure that the articles reflect a wide range of topics regarding journal scopes; to do a fair, scientific, fast, as well as high quality peer-review process; to provide a wide and diverse geographical coverage of articles around the world; and to publish the articles having a trustable resource of scientific information for the audiences. The types of acceptable submissions include original article, review article, short communication, letter to the editor, case report, editorial, as well as book review. Journal of Food Quality and Hazards Control is an official journal of Research Center for Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.