城乡湖泊中无性蜗牛种群的区域表观遗传变异

IF 4.8 Q1 GENETICS & HEREDITY
Jennifer L. M. Thorson, Mark W. Smithson, Ingrid Sadler‐Riggleman, Daniel Beck, M. Dybdahl, M. Skinner
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引用次数: 13

摘要

摘要表观遗传变异有可能影响环境依赖性发育,并有助于对当地环境的表型反应。性生物的环境表观遗传学研究证实了通过表观遗传学变异做出反应的能力。在缺乏遗传变异的群体中,表观遗传学反应可能更为重要。先前对无性蜗牛Potamopyrgus antipodarum的研究表明,在比较湖泊种群和河流种群时,来自单一克隆谱系的不同种群在外壳表型和甲基化特征方面存在差异。在这里,我们研究了不同环境干扰和污染历史的湖泊之间的甲基化变化。蜗牛是从西北太平洋海岸一个更原始的乡村湖泊1(莱尔湖)和两个城市湖泊2(国会湖)和3(华盛顿湖)采集的。使用甲基化DNA免疫沉淀MeDIP,然后进行下一代测序,对每个样本群体的DNA甲基化进行评估。不同湖泊比较中鉴定的差异DNA甲基化区域(DMRs)表明,农村湖泊1和一个城市湖泊2之间以及两个城市湖泊2中和3之间的DMRs数量和变异较高,但农村湖泊1与城市湖泊3之间的变异有限。研究DMR的基因组特征和基因关联。这些湖泊种群中每一个种群之间存在特定地点的差异,这表明它们对各种环境因素有表观遗传学反应。研究结果并不支持地理距离对这些人群的影响。讨论了湖泊之间的扩散距离、种群历史、环境污染和稳定遗传的甲基化与环境触发的甲基化在产生观察到的表观遗传变异中的作用。观察结果支持表观遗传学改变可能与表型变异、环境因素和不同湖泊的历史有关的观点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regional epigenetic variation in asexual snail populations among urban and rural lakes
Abstract Epigenetic variation has the potential to influence environmentally dependent development and contribute to phenotypic responses to local environments. Environmental epigenetic studies of sexual organisms confirm the capacity to respond through epigenetic variation. An epigenetic response could be even more important in a population when genetic variation is lacking. A previous study of an asexual snail, Potamopyrgus antipodarum, demonstrated that different populations derived from a single clonal lineage differed in both shell phenotype and methylation signature when comparing lake versus river populations. Here, we examine methylation variation among lakes that differ in environmental disturbance and pollution histories. Snails were collected from a more pristine rural Lake 1 (Lake Lytle), and two urban lakes, Lake 2 (Capitol Lake) and Lake 3 (Lake Washington) on the Northwest Pacific coast. DNA methylation was assessed for each sample population using methylated DNA immunoprecipitation, MeDIP, followed by next-generation sequencing. The differential DNA methylation regions (DMRs) identified among the different lake comparisons suggested a higher number of DMRs and variation between rural Lake 1 and one urban Lake 2, and between the two urban Lakes 2 and 3, but limited variation between the rural Lake 1 and urban Lake 3. DMR genomic characteristics and gene associations were investigated. The presence of site-specific differences between each of these lake populations suggest an epigenetic response to varied environmental factors. The results do not support an effect of geographic distance in these populations. The role of dispersal distance among lakes, population history, environmental pollution and stably inherited methylation versus environmentally triggered methylation in producing the observed epigenetic variation are discussed. Observations support the proposal that epigenetic alterations may associate with phenotypic variation and environmental factors and history of the different lakes.
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来源期刊
Environmental Epigenetics
Environmental Epigenetics GENETICS & HEREDITY-
CiteScore
6.50
自引率
5.30%
发文量
0
审稿时长
17 weeks
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