Diagnosis and Inhibition of the Virulence Factor Phaseolenone of the Pathogenic Fungus Macrophomina phaseolina Using Some Chemical and Biological Methods

Al-Jbory, A.A.A. and A.A Hasan. 2021. Diagnosis and Inhibition of the Virulence Factor Phaseolenone of the Pathogenic Fungus Macrophomina phaseolina Using Some Chemical and Biological Methods. Arab Journal of Plant Protection, 39(4): 252-256. https://doi.org/10.22268/AJPP-039.4.252256 Results obtained in this study confirmed the efficacy of some chemical and biological factors in reducing the concentration of Phaseolenone toxin produced by the fungal pathogen Macrophomina phaseolina grown on potato dextrose broth medium. The toxin concentrations were measured in different treatments using high performance liquid chromatography (HPLC). The citric acid treatment at 5% concentration surpassed the other treatments (lactic acid, acetic acid, SDS salts, EDTA, bacterial filtrates of Pseudomonas fluorescens and Bacillus subtilis). Citric acid treatment reduced by 5% the toxin concentration to 1.74 microgram/ml compared to 6.94 microgram/ml for the control treatment. This reduction in Phaseolenone concentration was also noticed through the reduction of the area under the curve which amounted to 1.684% for the citric acid treatment compared with 6.814% for the control treatment. Keywords: Phaseolenone, Macrophomina Phaseolina, charcoal rot, mycotoxin.