妊娠期糖尿病妇女环状RNA表达谱的嵌套病例对照研究

Q4 Medicine

中华围产医学杂志 Pub Date : 2019-04-16 DOI:10.3760/CMA.J.ISSN.1007-9408.2019.04.009

Ruihua Chen, Hongling Yang, R. Chen,, Jun Ma, Y. Long, Chunyan Zhu

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引用次数: 0

摘要

目的应用基因芯片技术研究妊娠期糖尿病（GDM）孕妇外周血圆环核糖核酸（circRNA）的表达，为研究GDM的发病机制提供依据。方法采用前瞻性队列嵌套病例对照研究，选取2014年9月至2016年4月在广州市妇幼保健中心分娩的018名孕妇。记录产前检查信息，并对分娩结果进行随访。6名被诊断为GDM的孕妇被选为GDM组。选择6例正常孕妇作为对照组，采用1∶1的方法采集血样。孕周差异小于7天，既往妊娠次数小于2次，既往分娩次数差异与对照组相同。应用染色体微阵列技术分析circRNA在母体外周血中的表达，并用GO分析其功能和调控网络预测(http://www.geneontology.org/)，KEGG通路(http://www.genome.jp/kegg/pathway.html)和CircNet(http://circnet.mbc.nctu.edu.tw/)。数据分析采用t检验。结果与正常孕妇相比，GDM妇女共有2678个circRNA差异表达>2.0倍，其中1 532个上调，1 146个下调。功能分析表明，上调的circRNA在胰岛素反应、基因沉默调节、胰高血糖素反应和细胞衰老的生物学过程中富集。信号通路分析表明circRNA参与胰岛素通路。以has_circ_0042852和has_circ _0004001为中心，预测了可能的GDM相关调控网络。结论GDM妇女外周血中含有丰富的circRNAs，可能参与许多生物学过程、胰岛素信号通路，并可能诱导GDM相关的调控网络。关键词：糖尿病，妊娠期；RNA；基因表达谱；生物标志物

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Circular RNA expression profiles in women with gestational diabetes mellitus: a nested case-control study

Objective To investigate the expression of circular RNA (circRNA) in peripheral blood of gravidas with gestational diabetes mellitus (GDM) using gene chip technology, and to provide evidence for studying pathogenesis of GDM. Methods A prospective cohort-based nested case-control study was used to select 1 018 pregnant women who were examined and delivered in Guangzhou Women and Children's Medical Center from September 2014 to April 2016. The information of prenatal examination was recorded and the outcome of delivery was followed up. Six pregnant women diagnosed as GDM were selected as the GDM group. Six normal pregnant women who were collected blood samples by 1∶1 were selected as the control group. The difference of gestational week was less than 7 days, the number of previous pregnancies was less than 2 times, and the difference of previous delivery times as same as the control group. The expression of circRNA in maternal peripheral blood was analyzed by chromosome microarray technique, and the function and the regulation network forecast were analyzed by GO (http://www.geneontology.org/), KEGG PATHWAY (http://www.genome.jp/kegg/pathway.html) and CircNet(http://circnet.mbc.nctu.edu.tw/). t-test was used for data analysis. Results Compared to the normal pregnant women, 2 678 circRNAs were identified to be differentially expressed >2.0 times in GDM women, among which 1 532 were up-regulated and 1 146 were down-regulated. Functional analysis showed that the up-regulated circRNA was enriched in the biological processes of insulin response, gene silencing regulation, glucagon response and cell senescence. Signal pathway analysis showed that circRNA involved in insulin pathway. Taking has_circ_0042852 and has_circ_0004001 as center, the possible GDM-related regulatory networks were predicted. Conclusions The peripheral blood of GDM women is rich in circRNAs, which might involve in many biological processes, insulin signaling pathway and possibly induding GDM-related regulatory networks. Key words: Diabetes, gestational; RNA; Gene expression profiling; Biomarkers

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来源期刊

中华围产医学杂志 Medicine-Obstetrics and Gynecology

CiteScore

0.70

自引率

0.00%

发文量

4446

期刊介绍： Chinese Journal of Perinatal Medicine was founded in May 1998. It is one of the journals of the Chinese Medical Association, which is supervised by the China Association for Science and Technology, sponsored by the Chinese Medical Association, and hosted by Peking University First Hospital. Perinatal medicine is a new discipline jointly studied by obstetrics and neonatology. The purpose of this journal is to "prenatal and postnatal care, improve the quality of the newborn population, and ensure the safety and health of mothers and infants". It reflects the new theories, new technologies, and new progress in perinatal medicine in related disciplines such as basic, clinical and preventive medicine, genetics, and sociology. It aims to provide a window and platform for academic exchanges, information transmission, and understanding of the development trends of domestic and foreign perinatal medicine for the majority of perinatal medicine workers in my country.