表皮细胞悬浮液在烧伤创面处理中的应用:一项初步研究

B. Mishra, V. Singh, C. Arora
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引用次数: 0

摘要

简介:尽早用植皮覆盖烧伤创面是一种标准做法。供体皮肤的缺乏严重限制了大面积烧伤的及时覆盖。研究表明,这些自体细胞通过减少宿主细胞侵入伤口组织所需的时间和通过早期合成新皮肤来增强伤口愈合过程。这种酶消化小皮肤移植物、离心悬浮细胞和将表皮细胞喷洒在烧伤原发区域的技术可以实现早期愈合。目的:本研究旨在评估表皮细胞悬液作为烧伤创面覆盖物的可行性和作用。材料和方法:本研究在印度一家三级医院的烧伤中心进行,为期1年。有烧伤后原始区域的患者也包括在内。在患者中发现了两个大小相似的位点。在一个部位应用细胞悬浮液,而其他部位则用传统敷料进行管理。采用小块离体皮片(SSG)制备细胞悬液。在麻醉下,从健康供体部位采集所需大小的SSG。进行移植物的胰蛋白酶化;表皮与真皮层分离。表皮被切成小块,加入患者血清并离心。制作表皮细胞悬浮液,并使用倒置显微镜确认细胞的活力。同时,用碳酸氢钠溶液彻底清洗伤口。在第1、3和5天施用新制备的角质形成细胞悬浮液。对照侧遵循类似的标准敷料方案。根据第7天和第14天再髓化的百分比进行伤口评估。结果:28名患者(18名男性和10名女性,平均年龄34岁)被纳入研究。将细胞悬浮液喷洒在4735cm2的面积上。伤口大小在第10天和第14天分别减少到2247cm2和923cm2。而对照部位的伤口在第7天和第14天分别减少到3882cm2和2134cm2。发现细胞悬浮液和对照处理区域的差异是显著的。结论:角质形成细胞悬液在烧伤中是可行的,可促进创面愈合。然而,还需要更大规模的研究来标准化和验证其在严重烧伤中的使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Use of epidermal cell suspension in burns wound management: A pilot study
Introduction: It is a standard practice to cover the burn wounds with skin grafts at the earliest. Deficiency of donor skin seriously limits timely cover of extensive burns. Studies have shown that these autologous cells enhance the wound-healing process by reducing the time needed for the host cells to invade the wound tissue and by early synthesis of new skin this technique of enzymatically digesting small skin grafts, centrifuging the suspended cells and spraying the epidermal cells over burn raw areas may allow early healing. Aims and Objective: The aim of this study is to assess feasibility and role of epidermal cell suspension as wound cover in burns. Materials and Methods: This study was conducted at the burn center of a tertiary Hospital in India over a period of 1 year. Patients with postburn raw areas were included. Similar size two sites were identified in the patients. On one site cell suspension was applied, while other areas were managed with conventional dressings. Small piece of split-skin graft (SSG) was used to make cell suspension. Under anesthesia, required size of SSG was harvested from healthy donor site. Trypsinization of graft was done; epidermis was separated from the dermis. The epidermis was cut into tiny pieces, and patient's serum was added and centrifuged. Epidermal cell suspension was made, and viability of cells is confirmed using an inverted microscope. Meanwhile, the wound is thoroughly cleaned by soda bicarbonate solution. The freshly prepared keratinocytes suspension was applied on day 1, 3, and 5. Similar standard dressing protocol was followed on the control side. Wound assessment was done based on the percentage of reepithelization on the 7th and 14th day. Results: Twenty-eight patients (18 males and 10 females with a mean age of 34 years) were included the study. Cell suspension was sprayed on 4735 cm2 area. Wound size reduced to 2247 cm2 and 923 cm2 on the 10th and 14th day, respectively. While, at control site wound reduced to 3882 cm2 and 2134 cm2 on the 7th and 14th day, respectively. The difference in cell suspension and control treated area was found to be significant. Conclusion: Keratinocytes cell suspension is feasible in burns and improves the healing of wounds. However, larger studies are required to standardize and validate its use in major burns.
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