R. J. Barrón-Rodríguez, F. Chávez-Maya, E. Loza-Rubio, G. García-Espinosa
{"title":"从墨西哥临时人工池塘中分离和鉴定甲型流感病毒(H6N2)","authors":"R. J. Barrón-Rodríguez, F. Chávez-Maya, E. Loza-Rubio, G. García-Espinosa","doi":"10.21753/VMOA.5.2.475","DOIUrl":null,"url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Barron-Rodriguez RJ, Chavez-Maya F, Loza-Rubio E, Garcia-Espinosa G. Isolation and characterization of influenza A virus (H6N2) from a temporary artificial pond in Mexico. Veterinaria Mexico OA. 2018;5(2). doi: 10.21753/5.2.475. . Most epidemiological surveillance studies of the influenza A virus (IAV) have focused on the isolation and detection of the virus in wild birds. However, there are limited descriptions of both the wild duck population and the purpose and size of the aquatic habitats where viruses have been detected or isolated. The objective of this study was to determine if a pond of 16 hectares (39.536 acres), used for agricultural and fishing purposes and visited by approximately 9000 wild migratory ducks consisting of nine different species during the wintering stay, is suitable to support the isolation of IAV. One influenza A virus was isolated from Pekin ducks used as sentinels during the wintering stay season from September 2007 to March 2008. Only one IAV subtype was isolated from 9 of the 88 samples collected from the sentinel ducks over seven months, and the molecular characterization of this isolate revealed an H6N2 virus subtype. Based on this information, it is suggested that a pond such as the one in this study provides a suitable biological setting to support the presence of IAV, but the minimum biological environment to isolate the influenza A virus is still unknown. Figure 1. Location of the artificial pond in the State of Mexico with 19°24’22” N, 99°41’36” W coordinates and the routes of migratory birds in America (Google Earth).","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2000,"publicationDate":"2018-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.5.2.475","citationCount":"1","resultStr":"{\"title\":\"Isolation and characterization of influenza A virus (H6N2) from a temporary artificial pond in Mexico\",\"authors\":\"R. J. Barrón-Rodríguez, F. Chávez-Maya, E. Loza-Rubio, G. García-Espinosa\",\"doi\":\"10.21753/VMOA.5.2.475\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Barron-Rodriguez RJ, Chavez-Maya F, Loza-Rubio E, Garcia-Espinosa G. Isolation and characterization of influenza A virus (H6N2) from a temporary artificial pond in Mexico. Veterinaria Mexico OA. 2018;5(2). doi: 10.21753/5.2.475. . Most epidemiological surveillance studies of the influenza A virus (IAV) have focused on the isolation and detection of the virus in wild birds. However, there are limited descriptions of both the wild duck population and the purpose and size of the aquatic habitats where viruses have been detected or isolated. The objective of this study was to determine if a pond of 16 hectares (39.536 acres), used for agricultural and fishing purposes and visited by approximately 9000 wild migratory ducks consisting of nine different species during the wintering stay, is suitable to support the isolation of IAV. One influenza A virus was isolated from Pekin ducks used as sentinels during the wintering stay season from September 2007 to March 2008. Only one IAV subtype was isolated from 9 of the 88 samples collected from the sentinel ducks over seven months, and the molecular characterization of this isolate revealed an H6N2 virus subtype. Based on this information, it is suggested that a pond such as the one in this study provides a suitable biological setting to support the presence of IAV, but the minimum biological environment to isolate the influenza A virus is still unknown. Figure 1. Location of the artificial pond in the State of Mexico with 19°24’22” N, 99°41’36” W coordinates and the routes of migratory birds in America (Google Earth).\",\"PeriodicalId\":49387,\"journal\":{\"name\":\"Veterinaria Mexico\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2018-04-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.21753/VMOA.5.2.475\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinaria Mexico\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.21753/VMOA.5.2.475\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Veterinary\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinaria Mexico","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.21753/VMOA.5.2.475","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Veterinary","Score":null,"Total":0}
Isolation and characterization of influenza A virus (H6N2) from a temporary artificial pond in Mexico
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Barron-Rodriguez RJ, Chavez-Maya F, Loza-Rubio E, Garcia-Espinosa G. Isolation and characterization of influenza A virus (H6N2) from a temporary artificial pond in Mexico. Veterinaria Mexico OA. 2018;5(2). doi: 10.21753/5.2.475. . Most epidemiological surveillance studies of the influenza A virus (IAV) have focused on the isolation and detection of the virus in wild birds. However, there are limited descriptions of both the wild duck population and the purpose and size of the aquatic habitats where viruses have been detected or isolated. The objective of this study was to determine if a pond of 16 hectares (39.536 acres), used for agricultural and fishing purposes and visited by approximately 9000 wild migratory ducks consisting of nine different species during the wintering stay, is suitable to support the isolation of IAV. One influenza A virus was isolated from Pekin ducks used as sentinels during the wintering stay season from September 2007 to March 2008. Only one IAV subtype was isolated from 9 of the 88 samples collected from the sentinel ducks over seven months, and the molecular characterization of this isolate revealed an H6N2 virus subtype. Based on this information, it is suggested that a pond such as the one in this study provides a suitable biological setting to support the presence of IAV, but the minimum biological environment to isolate the influenza A virus is still unknown. Figure 1. Location of the artificial pond in the State of Mexico with 19°24’22” N, 99°41’36” W coordinates and the routes of migratory birds in America (Google Earth).
期刊介绍:
Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges.
This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).