新型丙型肝炎病毒繁殖抑制剂N-硬脂酰乙醇胺

Q4 Biochemistry, Genetics and Molecular Biology

Biopolymers and Cell Pub Date : 2021-06-30 DOI:10.7124/bc.000a54

N. Hula, A. Chumak, A. Berdyshev, G. V. Kosiakova, T. M. Goridko, O. F. Meged', V. S. Asmolkova, Yu. I. Porva, S. Rybalko, S. T. Dyadun, D. Starosyla, O. Deryabin

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引用次数: 0

摘要

目标内源性模拟大麻酚化合物N-硬脂酰乙醇胺（NSE）对丙型肝炎病毒（HCV）繁殖影响的研究。方法。替代HCV的模型是牛腹泻病毒；细胞培养模型是用人HCV的cDNA转染的细胞，并且已经使用了分子对接。后果体外研究表明，NSE有效地抑制了MDBK细胞和转染的Jurkat细胞中替代HCV的繁殖。分子对接表明，NSE可以与NS3丝氨酸蛋白酶和HCV NS5B聚合酶的活性中心结合，并对其活性具有抑制作用。结论。所获得的数据证实，使用NSE对开发

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N-stearoylethanolamine - a new inhibitor of the hepatitis C virus reproduction

Aim. The study of the effect of endogenous cannabimimetic compound - N-stearoylethanolamine (NSE) on the hepatitis C virus (HCV) reproduction. Methods. The model of the surrogate HCV is a bovine diarrhea virus; cell culture model is cells transfected with cDNA of the human HCV and molecular docking has been used. Results. In vitro studies showed that NSE effectively inhibited the reproduction of a surrogate HCV in both MDBK cells and transfected Jurkat cells. Molecular docking suggested that NSE can bind to the active centers of both NS3 serine protease and HCV NS5B-polymerase and has an inhibitory effect on their activity. Conclusions. The obtained data confirm that using NSE is promising for the develop-ment

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来源期刊

Biopolymers and Cell Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： “Biopolymer and cell” is published since 1985 at the Institute of Molecular Biology and Genetics NAS of Ukraine under the supervision of the National Academy of Sciences of Ukraine. Our journal covers a wide scope of problems related to molecular biology and genetics including structural and functional genomics, transcriptomics, proteomics, bioinformatics, biomedicine, molecular enzymology, molecular virology and immunology, theoretical bases of biotechnology, physics and physical chemistry of proteins and nucleic acids and bioorganic chemistry.