Xiangyu Xu, Jiayi Xu, Jie Liu, Chaohui Jiang, Liangfei Tian, Yingke Xu, Dechang Li, B. Ji
{"title":"细胞集体迁移指状结构的细胞力学","authors":"Xiangyu Xu, Jiayi Xu, Jie Liu, Chaohui Jiang, Liangfei Tian, Yingke Xu, Dechang Li, B. Ji","doi":"10.1115/1.4063217","DOIUrl":null,"url":null,"abstract":"\n Finger-like structures take crucial roles in migration behaviors of collective cells. However, the mechanics of the finger-like structure has not been fully understood. Here, we constructed a two-dimensional collective cell migration model, and quantitatively analyzed the cellular mechanics of finger-like structures during the collective cell migration through experimental study and numerical simulation. We found that substrate stiffness, cell density, cell prestress, and mechanical loading significantly influence the generation and behaviors of the finger-like structures through regulating the lamellipodia spreading area, cellular traction force, and collectivity of cell motility. We showed that the regions with the higher maximum principal stress tend to produce larger finger-like structures. Increasing the spreading area of lamellipodia and the velocity of leader cells could promote the generation of higher finger-like structures. For a quantitative understanding of the mechanisms of the effects of these mechanical factors, we adopted a coarse-grained cell model based on the traction-distance law. Our numerical simulation recapitulated the cell velocity distribution, cell motility integrity, cell polarization, and the stress distribution in the cell layer observed in the experiment. These analyses reveal the cellular mechanics of the finger-like structure and its roles in collective cell migration. This study provides useful insights into the collective cell behaviors in tissue engineering and regenerative medicine for biomedical applications.","PeriodicalId":54880,"journal":{"name":"Journal of Applied Mechanics-Transactions of the Asme","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2023-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cellular mechanics of finger-like structures of collective cell migration\",\"authors\":\"Xiangyu Xu, Jiayi Xu, Jie Liu, Chaohui Jiang, Liangfei Tian, Yingke Xu, Dechang Li, B. Ji\",\"doi\":\"10.1115/1.4063217\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n Finger-like structures take crucial roles in migration behaviors of collective cells. However, the mechanics of the finger-like structure has not been fully understood. Here, we constructed a two-dimensional collective cell migration model, and quantitatively analyzed the cellular mechanics of finger-like structures during the collective cell migration through experimental study and numerical simulation. We found that substrate stiffness, cell density, cell prestress, and mechanical loading significantly influence the generation and behaviors of the finger-like structures through regulating the lamellipodia spreading area, cellular traction force, and collectivity of cell motility. We showed that the regions with the higher maximum principal stress tend to produce larger finger-like structures. Increasing the spreading area of lamellipodia and the velocity of leader cells could promote the generation of higher finger-like structures. For a quantitative understanding of the mechanisms of the effects of these mechanical factors, we adopted a coarse-grained cell model based on the traction-distance law. Our numerical simulation recapitulated the cell velocity distribution, cell motility integrity, cell polarization, and the stress distribution in the cell layer observed in the experiment. These analyses reveal the cellular mechanics of the finger-like structure and its roles in collective cell migration. This study provides useful insights into the collective cell behaviors in tissue engineering and regenerative medicine for biomedical applications.\",\"PeriodicalId\":54880,\"journal\":{\"name\":\"Journal of Applied Mechanics-Transactions of the Asme\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2023-08-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Mechanics-Transactions of the Asme\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1115/1.4063217\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MECHANICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Mechanics-Transactions of the Asme","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1115/1.4063217","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MECHANICS","Score":null,"Total":0}
Cellular mechanics of finger-like structures of collective cell migration
Finger-like structures take crucial roles in migration behaviors of collective cells. However, the mechanics of the finger-like structure has not been fully understood. Here, we constructed a two-dimensional collective cell migration model, and quantitatively analyzed the cellular mechanics of finger-like structures during the collective cell migration through experimental study and numerical simulation. We found that substrate stiffness, cell density, cell prestress, and mechanical loading significantly influence the generation and behaviors of the finger-like structures through regulating the lamellipodia spreading area, cellular traction force, and collectivity of cell motility. We showed that the regions with the higher maximum principal stress tend to produce larger finger-like structures. Increasing the spreading area of lamellipodia and the velocity of leader cells could promote the generation of higher finger-like structures. For a quantitative understanding of the mechanisms of the effects of these mechanical factors, we adopted a coarse-grained cell model based on the traction-distance law. Our numerical simulation recapitulated the cell velocity distribution, cell motility integrity, cell polarization, and the stress distribution in the cell layer observed in the experiment. These analyses reveal the cellular mechanics of the finger-like structure and its roles in collective cell migration. This study provides useful insights into the collective cell behaviors in tissue engineering and regenerative medicine for biomedical applications.
期刊介绍:
All areas of theoretical and applied mechanics including, but not limited to: Aerodynamics; Aeroelasticity; Biomechanics; Boundary layers; Composite materials; Computational mechanics; Constitutive modeling of materials; Dynamics; Elasticity; Experimental mechanics; Flow and fracture; Heat transport in fluid flows; Hydraulics; Impact; Internal flow; Mechanical properties of materials; Mechanics of shocks; Micromechanics; Nanomechanics; Plasticity; Stress analysis; Structures; Thermodynamics of materials and in flowing fluids; Thermo-mechanics; Turbulence; Vibration; Wave propagation