转基因马铃薯（Solanum tuberosum L.）品种IPB CP3的获得

Q4 Environmental Science

Indonesian Journal of Biotechnology Pub Date : 2021-03-30 DOI:10.22146/IJBIOTECH.61682

Pasmawati Pasmawati, A. Tjahjoleksono, S. Suharsono

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引用次数: 1

摘要

青枯菌引起的青萎病是马铃薯生产中最重要的细菌性病害之一。本研究旨在获得转基因马铃薯（Solanum tuberosum L.）品种IPB CP3，该品种含有编码溶菌酶C型的LYZ‐C基因，对青枯菌引起的细菌性疾病具有抗性。根癌农杆菌LBA4404对124个节间外植体进行遗传转化，转化效率约为47.58%，再生效率约为30.51%。基因整合分析表明，16个克隆被确认为含有LYZ-C基因的转基因克隆。对三个转基因克隆对青枯菌的抗性分析表明，所有三个转基因无性系都比非转基因无性系更具抗性。这一结果表明，转基因马铃薯基因组中整合的LYZ‐C基因增加了马铃薯植株对青枯菌的抗性。我们获得了两个被认为对青萎病具有抗性的转基因克隆。

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Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease

Bacterial wilt caused by Ralstonia solanacearum is one of the most important bacterial diseases in potato production. This study aimed to obtain the transgenic potato ( Solanum tuberosum L.) cultivar IPB CP3, containing LYZ‐C gene encoding for lysozyme type C, resistant to bacterial disease caused by R. solanacearum . Genetic transformation using Agrobacterium tumefaciens LBA4404 to 124 internode explants resulted in the transformation efficiency of about 47.58% with a regeneration efficiency of approximately 30.51%. Gene integration analysis showed that 16 clones were confirmed as transgenic clones containing the LYZ‐C gene. Analysis of resistance to R. solanacearum of three transgenic clones showed that all three transgenic clones were more resistant than a non‐transgenic one. This result showed that the LYZ‐C gene integrated in the genome of transgenic potato increased the resistance of potato plants to R. solanacearum . We obtained two transgenic clones considered resistant to bacterial wilt disease.

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来源期刊

Indonesian Journal of Biotechnology Environmental Science-Environmental Science (miscellaneous)

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

12 weeks