小麦-黑麦易位(WRT) 1AL/1RS和1BL/1RS在软质冬小麦育种中的利用效率

M. Lytvynenko, E. Holub, T. Khomenko
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引用次数: 0

摘要

意图为了测定WRT 1AL.1RS和1BL.1RS对重组品系产量、植物生产力要素和质量指标的遗传效应,确定在乌克兰草原地区土壤空气干旱的情况下,使用每种WRT在这些性状上创造更完美的软冬小麦品种的有效性,并开发育种技术,以减少易位的负面影响,从而生产出具有高质量指数的有价值和结实小麦的基因型。方法。田间试验、种内杂交、田间育种材料评价、谷物烘焙质量指标的实验室测定方法、备用蛋白质电泳、统计学。后果在乌克兰南部干旱条件下,在育种过程的大型实验材料上,确定了1AL.1RS对重组品系产量和植物生产力的主要因素的积极影响,这是在这种换位对耐旱性和耐热性同时产生积极影响的背景下表现出来的。1BL.1RS在该地区小麦育种中的应用前景不大。研究表明,通过杂交将1AL.1RS、1BL.1RS易位引入软冬小麦的局部基因库,可以改变小麦的品质指标。特别是,在1BL.1RS易位的影响下,蛋白质含量往往会更显著地增加。研究表明,获得高产和足够烘焙性能水平(不低于有价值和结实的小麦)的重组品系的频率很低(1,7–6,1%),但具有1AL.1RS的渗入品系在这一参数上具有优势。利用WRT与对烘烤性能具有高度积极影响的等位基因杂交等遗传因素,以及在有和无WRT的基因型组成中产生异质性,可以有目的地减少WRT对软冬小麦品质的负面影响,创造出具有有价值、结实小麦品质参数的品种。结论。总的来说,所取得的结果有理由断言,WRT 1AL.1RS的使用是在乌克兰南部干旱条件下进一步育种提高软冬小麦品种遗传能力的一个有希望的方向。由于使用1AL.1RS WRT对材料进行了全周期育种,创建了一系列软冬小麦品种“Zhytnytsia odeska”、“Oktava odeska’”、“Liha odeska‘”、“Duma odeska“”、“Versiia odeska'”,使产量达到标准提高了10-15%,并被列入乌克兰和摩尔多瓦国家登记册。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Efficiency of using of wheat-rye translocations (WRT) 1AL/1RS and 1BL/1RS in soft winter wheat breeding
Purpose. To determine the genetic effects of WRT 1AL.1RS and 1BL.1RS on the yield, plant productivity elements and quality indices of recombinant lines, to determine the effectiveness of using each of the WRT for creating more perfect varieties of soft winter wheat in these traits under soil-air drought in the steppe zone of Ukraine and development of breeding techniques to reduce the negative effects of translocation to produce genotypes with high quality indices of valuable and strong wheat grain. Methods. Field experiments, intraspecific hybridization, evaluation of breeding material in the field, methods of laboratory determination of baking quality indices of grain, electrophoresis of spare proteins, statistical. Results. Under arid conditions of the South of Ukraine on the large experimental material of breeding process, a positive effect of 1AL.1RS on the yield of recombinant lines and the main elements of plant productivity were determined, which was manifested against the background of simultaneous positive effect of this transposition on the drought and heat tolerance. The use of 1BL.1RS in wheat breeding in this region is less promising technique. It has been determined that introduction of 1AL.1RS, 1BL.1RS translocations into local gene pool of soft winter wheat by hybridization changes the grain quality indices. In particular, the protein content tends to increase more significantly under the influence of 1BL.1RS translocation. It has been shown that the frequency of obtaining recombinant lines which combine high yield and sufficient level of baking properties (not lower than valuable and strong wheat) is quite low (1,7–6,1%), but introgressive lines with 1AL.1RS have the advantages in this parameter. Using such genetic factors as hybridization combining WRT with alleles with high positive effect on baking properties, and also creating heterogeneity in the composition of genotypes with and without WRT, one can purposefully reduce the negative impact of WRT on the quality of soft winter wheat grain and create varieties with quality parameters of valuable and strong wheat. Conclusions. In general, the results achieved give reason to assert that the use of WRT 1AL.1RS is a promising direction for further breeding increase of genetic capacity of soft winter wheat varieties in the arid conditions of the South of Ukraine. As a result of full cycle of breeding process on the material with 1AL.1RS WRT a series of varieties of soft winter wheat ‘Zhytnytsia odeska’, ‘Oktava odeska’, ‘Liha odeska’, ‘Duma odeska’, ‘Versiia odeska’, providing 10 – 15% increase in yield to standards was created and included in the State Register of Ukraine and Moldova.
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