甲状腺功能减退背景下Guerin’s癌大鼠下丘脑-垂体-性腺轴连接紊乱

O. Kit, V. Bandovkina, E. Frantsiyants, I. Kaplieva, E. Surikova, I. Neskubina, N. Cheryarina, Y. Pogorelova, L. K. Trepitaki, L. Nemashkalova, T. Moiseenko, A. Arakelova, M. Adamyan, N. Chernikova, Aina M. Salatova, Irina, V. Kaplieva, D. Natalia, Cheryarina, A. Lyudmila, Nemashkalova, L. Meri, Adamyan
{"title":"甲状腺功能减退背景下Guerin’s癌大鼠下丘脑-垂体-性腺轴连接紊乱","authors":"O. Kit, V. Bandovkina, E. Frantsiyants, I. Kaplieva, E. Surikova, I. Neskubina, N. Cheryarina, Y. Pogorelova, L. K. Trepitaki, L. Nemashkalova, T. Moiseenko, A. Arakelova, M. Adamyan, N. Chernikova, Aina M. Salatova, Irina, V. Kaplieva, D. Natalia, Cheryarina, A. Lyudmila, Nemashkalova, L. Meri, Adamyan","doi":"10.18137/cardiometry.2023.27.1219","DOIUrl":null,"url":null,"abstract":"Aims: to study the content of regulatory peptides in the hypothalamus and pituitary gland, as well as sex steroids in the gonads in rats of both sexes with Guerin’s carcinoma against the background of hypothyroidism. Materials and methods. Our experiment was performed in white outbred rats of both sexes with induced hypothyroidism, against the background of which Guerin’s carcinoma was transplanted. In 1% homogenates of the pituitary and the hypothalamus and in 10% homogenates of the gonads in the animals from the main and reference groups, the levels of GnRH (Casabio, China) were determined by the ELISA method using standard kits, and the content of LH, FSH, estradiol (E2), testosterone (T) and progesterone (P4) by the RIA method (Immunotech, Czech Republic). Statistical analysis of the results was performed using the Statistica 10.0 software package. Results. In females of the main group, the level of GnRH in the hypothalamus increased by 1.6 times, but the content of LH and FSH in the pituitary gland decreased, while in males the content of GnRH decreased by 1.4 times and FSH by 1.6 times compared with the intact animals. In the gonads of females of the main group, the content of E2 and T decreased by 1.3 times, but the level of P4 increased by 4.7 times, while in males the T production decreased by 7.1 times. Hypothyroidism, against the background of which Guerin’s carcinoma developed, had a significant effect both on the central links of the HPG axis and the peripheral ones, producing an additional hormonal imbalance in sex steroids","PeriodicalId":41726,"journal":{"name":"Cardiometry","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Disorders in connections of the hypothalamic-pituitarygonadal axis in rats with Guerin’s carcinoma against the background of induced hypothyroidism\",\"authors\":\"O. Kit, V. Bandovkina, E. Frantsiyants, I. Kaplieva, E. Surikova, I. Neskubina, N. Cheryarina, Y. Pogorelova, L. K. Trepitaki, L. Nemashkalova, T. Moiseenko, A. Arakelova, M. Adamyan, N. Chernikova, Aina M. Salatova, Irina, V. Kaplieva, D. Natalia, Cheryarina, A. Lyudmila, Nemashkalova, L. Meri, Adamyan\",\"doi\":\"10.18137/cardiometry.2023.27.1219\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aims: to study the content of regulatory peptides in the hypothalamus and pituitary gland, as well as sex steroids in the gonads in rats of both sexes with Guerin’s carcinoma against the background of hypothyroidism. Materials and methods. Our experiment was performed in white outbred rats of both sexes with induced hypothyroidism, against the background of which Guerin’s carcinoma was transplanted. In 1% homogenates of the pituitary and the hypothalamus and in 10% homogenates of the gonads in the animals from the main and reference groups, the levels of GnRH (Casabio, China) were determined by the ELISA method using standard kits, and the content of LH, FSH, estradiol (E2), testosterone (T) and progesterone (P4) by the RIA method (Immunotech, Czech Republic). Statistical analysis of the results was performed using the Statistica 10.0 software package. Results. In females of the main group, the level of GnRH in the hypothalamus increased by 1.6 times, but the content of LH and FSH in the pituitary gland decreased, while in males the content of GnRH decreased by 1.4 times and FSH by 1.6 times compared with the intact animals. In the gonads of females of the main group, the content of E2 and T decreased by 1.3 times, but the level of P4 increased by 4.7 times, while in males the T production decreased by 7.1 times. Hypothyroidism, against the background of which Guerin’s carcinoma developed, had a significant effect both on the central links of the HPG axis and the peripheral ones, producing an additional hormonal imbalance in sex steroids\",\"PeriodicalId\":41726,\"journal\":{\"name\":\"Cardiometry\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-05-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cardiometry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18137/cardiometry.2023.27.1219\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cardiometry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18137/cardiometry.2023.27.1219","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

目的:研究甲状腺功能减退背景下Guerin’s癌大鼠下丘脑和垂体调节肽以及性腺性激素的含量。材料和方法。我们的实验是在患有甲状腺功能减退症的雄性和雌性的白色大鼠身上进行的,背景是移植了格林氏癌。在主要组和参考组动物的1%垂体和下丘脑匀浆以及10%性腺匀浆中,使用标准试剂盒通过ELISA法测定GnRH水平(Casabio,中国),并通过RIA法测定LH、FSH、雌二醇(E2)、睾酮(T)和孕酮(P4)含量(Immunotech,捷克共和国)。使用Statistica 10.0软件包对结果进行统计分析。后果主要组雌性下丘脑GnRH水平比正常组增加1.6倍,但垂体LH和FSH含量下降,雄性GnRH含量下降1.4倍,FSH下降1.6倍。在主要群体的雌性性腺中,E2和T的含量下降了1.3倍,但P4的水平上升了4.7倍,而雄性的T产量下降了7.1倍。甲状腺功能减退症是在格林氏癌发生的背景下发生的,它对HPG轴的中心环节和外周环节都有显著影响,从而导致性类固醇激素的额外失衡
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Disorders in connections of the hypothalamic-pituitarygonadal axis in rats with Guerin’s carcinoma against the background of induced hypothyroidism
Aims: to study the content of regulatory peptides in the hypothalamus and pituitary gland, as well as sex steroids in the gonads in rats of both sexes with Guerin’s carcinoma against the background of hypothyroidism. Materials and methods. Our experiment was performed in white outbred rats of both sexes with induced hypothyroidism, against the background of which Guerin’s carcinoma was transplanted. In 1% homogenates of the pituitary and the hypothalamus and in 10% homogenates of the gonads in the animals from the main and reference groups, the levels of GnRH (Casabio, China) were determined by the ELISA method using standard kits, and the content of LH, FSH, estradiol (E2), testosterone (T) and progesterone (P4) by the RIA method (Immunotech, Czech Republic). Statistical analysis of the results was performed using the Statistica 10.0 software package. Results. In females of the main group, the level of GnRH in the hypothalamus increased by 1.6 times, but the content of LH and FSH in the pituitary gland decreased, while in males the content of GnRH decreased by 1.4 times and FSH by 1.6 times compared with the intact animals. In the gonads of females of the main group, the content of E2 and T decreased by 1.3 times, but the level of P4 increased by 4.7 times, while in males the T production decreased by 7.1 times. Hypothyroidism, against the background of which Guerin’s carcinoma developed, had a significant effect both on the central links of the HPG axis and the peripheral ones, producing an additional hormonal imbalance in sex steroids
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Cardiometry
Cardiometry MEDICAL LABORATORY TECHNOLOGY-
自引率
0.00%
发文量
0
审稿时长
6 weeks
期刊介绍: Cardiometry is an open access biannual electronic journal founded in 2012. It refers to medicine, particularly to cardiology, as well as oncocardiology and allied science of biophysics and medical equipment engineering. We publish mainly high quality original articles, reports, case reports, reviews and lectures in the field of the theory of cardiovascular system functioning, principles of cardiometry, its diagnostic methods, cardiovascular system therapy from the aspect of cardiometry, system and particular approaches to maintaining health, engineering peculiarities in cardiometry developing. The interdisciplinary areas of the journal are: hemodynamics, biophysics, biochemistry, metrology. The target audience of our Journal covers healthcare providers including cardiologists and general practitioners, bioengineers, biophysics, medical equipment, especially cardiology diagnostics device, developers, educators, nurses, healthcare decision-makers, people with cardiovascular diseases, cardiology and engineering universities and schools, state and private clinics. Cardiometry is aimed to provide a wide forum for exchange of information and public discussion on above scientific issues for the mentioned experts.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信