根管感染粪肠球菌噬菌体的分离与鉴定(体外研究)

IF 2.1 Q2 MEDICINE, GENERAL & INTERNAL
Maha F. Almelan, Uroba Khalid Abbas, Al-Zubidi M.
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引用次数: 0

摘要

引言和目的:粪肠球菌是一种革兰氏阳性细菌,栖息在人类和动物肠道及环境中。粪便大肠杆菌除了与人类的各种感染有关外,还被认为是与复发性根管治疗失败相关的最常见威胁。在这项研究中,我们旨在表征从慢性根管感染中分离的粪大肠杆菌菌株,并评估噬菌体消除粪大肠杆菌生物膜形成的能力。材料和方法:本研究涉及65份慢性根管感染样本,对粪肠球菌的患病率进行了研究。粪肠球菌的鉴定是通过生物化学测试和16S rRNA基因测序相结合的方法完成的。从牛粪样品中分离的噬菌体经过纯化,并根据其防止粪肠球菌在生物表面形成生物膜的能力进行评估。结果:在研究的65个慢性根管感染样本中,19个样本的粪便大肠杆菌检测呈阳性。粪便大肠杆菌菌株K3被发现具有万古霉素抗性。暴露于肠球菌噬菌体BAG1后,粪肠球菌菌株K3在非生物表面上的生物膜发育被证明大大减少。结论:本研究表明,粪便大肠杆菌噬菌体BAG1显著降低了粪便大肠杆菌生物膜在非生物表面的生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and characterization of bacteriophage targeting Enterococcus faecalis isolated from root canal infection (in vitro study)
Introduction and Aim: Enterococcus faecalis, a Gram-positive bacterium inhabits the human and animal intestinal tracts and the environment. E. faecalis in addition to being implicated in a wide variety of infections in humans is considered the most common threat associated with recurrent root canal treatment failures. In this investigation we aimed to characterize E. faecalis strains isolated from chronic endodontic infection and evaluate the effect of a bacteriophage for its capability to eliminate E. faecalis biofilm formation.   Materials and Methods: This study involved 65 chronic endodontic infection samples which were studied for the prevalence of Enterococcus faecalis. The identification of E. faecalis was accomplished by a combination of biochemical testing and the sequencing of the 16S rRNA gene. Phages isolated from cow dung samples were purified and evaluated based on their capability of preventing the development of biofilms by Enterococcus faecalis on biotic surfaces.   Results: Of the 65 chronic endodontic infection samples studied nineteen tested positive for the presence of E. faecalis. E. faecalis strain K3 was found to be vancomycin resistant. Biofilm development on abiotic surfaces by E. faecalis strain K3 was shown to be greatly reduced after exposure to the enterococcal phage BAG1.   Conclusion: This investigation shows that the E. faecalis bacteriophage BAG1 significantly decreases the growth of E. faecalis biofilm on abiotic surfaces.
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来源期刊
BioMedicine-Taiwan
BioMedicine-Taiwan MEDICINE, GENERAL & INTERNAL-
CiteScore
2.80
自引率
5.90%
发文量
21
审稿时长
24 weeks
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