二次代谢剖面,枣椰树提取物中的抗氧化剂和抗菌活性(凤梨属)。自由脂肪

Afrizal Afrizal, Aditya Perdana, S. Suryati
{"title":"二次代谢剖面,枣椰树提取物中的抗氧化剂和抗菌活性(凤梨属)。自由脂肪","authors":"Afrizal Afrizal, Aditya Perdana, S. Suryati","doi":"10.25077/jrk.v13i1.423","DOIUrl":null,"url":null,"abstract":"Dates are one of the plants that grow in the Middle East, one of them is the Golden Valley variety. Dates are usually consumed directly and can also be processed into date juice drinks. Dates refined process would produce waste in the form of date seeds are discarded and not processed into other products. This study used lipid-free Golden valley date seed extract to determine the secondary metabolite profile by using LC-MS/MS method, determining phenolic content using the Folin-Ciocalteau method, determining total flavonoids using the aluminium chloride method, determining antioxidant activity by DPPH method, and determination of antibacterial activity by the disc diffusion method. The results showed that the compounds identified from the lipid-free date seed extract were catechin-(4α→8)-catechin, bis((1S,2R,5S)-5-isopropyl-2,3-dimethylcyclo-hexyl)-2,6-dimethylpyridine-3,5-dicarboxylate, 3,5,6-trihydroxy-4',7-dimethoxyflavone, ethyl-2-amino-3-oxobutanoate, and bis(2-isopropyl-5-methylcyclohexyl)-2,6-dimethyl-1,4-dihydro-3,5-pyridine dicarboxylate. Lipid-free date seed extract contains total phenolic and flavonoid totals of 4364.704 mgGAE/100 grams of dried extract and 17200 mgQE/100 grams of dried extract. Its antioxidant activity is very strong in counteracting DPPH free radicals indicated by IC50 value of 10.1951 mg/L. Antibacterial activity shows strong inhibition of Staphylococcus aureus bacteria compared to inhibition of Escherichia coli bacteria that are categorized as weak.","PeriodicalId":33366,"journal":{"name":"Jurnal Riset Kimia","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Penentuan Profil Metabolit Sekunder, Aktivitas Antioksidan dan Antibakteri dari Ekstrak Biji Kurma (Phoenix dactylifera L.) Bebas Lipid\",\"authors\":\"Afrizal Afrizal, Aditya Perdana, S. Suryati\",\"doi\":\"10.25077/jrk.v13i1.423\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Dates are one of the plants that grow in the Middle East, one of them is the Golden Valley variety. Dates are usually consumed directly and can also be processed into date juice drinks. Dates refined process would produce waste in the form of date seeds are discarded and not processed into other products. This study used lipid-free Golden valley date seed extract to determine the secondary metabolite profile by using LC-MS/MS method, determining phenolic content using the Folin-Ciocalteau method, determining total flavonoids using the aluminium chloride method, determining antioxidant activity by DPPH method, and determination of antibacterial activity by the disc diffusion method. The results showed that the compounds identified from the lipid-free date seed extract were catechin-(4α→8)-catechin, bis((1S,2R,5S)-5-isopropyl-2,3-dimethylcyclo-hexyl)-2,6-dimethylpyridine-3,5-dicarboxylate, 3,5,6-trihydroxy-4',7-dimethoxyflavone, ethyl-2-amino-3-oxobutanoate, and bis(2-isopropyl-5-methylcyclohexyl)-2,6-dimethyl-1,4-dihydro-3,5-pyridine dicarboxylate. Lipid-free date seed extract contains total phenolic and flavonoid totals of 4364.704 mgGAE/100 grams of dried extract and 17200 mgQE/100 grams of dried extract. Its antioxidant activity is very strong in counteracting DPPH free radicals indicated by IC50 value of 10.1951 mg/L. Antibacterial activity shows strong inhibition of Staphylococcus aureus bacteria compared to inhibition of Escherichia coli bacteria that are categorized as weak.\",\"PeriodicalId\":33366,\"journal\":{\"name\":\"Jurnal Riset Kimia\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-03-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jurnal Riset Kimia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.25077/jrk.v13i1.423\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Riset Kimia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.25077/jrk.v13i1.423","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

枣子是一种生长在中东的植物,其中一种是金谷品种。枣通常直接食用,也可以加工成枣汁饮料。枣子精制过程中会产生废弃物,枣子被丢弃,不能加工成其他产品。本研究以无脂金谷枣籽提取物为原料,采用LC-MS/MS法测定其次生代谢产物谱,采用Folin-Ciocalteau法测定酚类含量,采用氯化铝法测定总黄酮含量,采用DPPH法测定其抗氧化活性,采用圆盘扩散法测定其抗菌活性。结果表明,从无脂枣籽提取物中分离得到的化合物为儿茶素-(4α→8)-儿茶素、二((1S,2R,5S)-5-异丙基-2,3-二甲基环己基)-2,6-二甲基吡啶-3,5-二羧酸盐、3,5,6-三羟基-4',7-二甲氧基黄酮、乙基-2-氨基-3-氧丁酸盐和二(2-异丙基-5-甲基环己基)-2,6-二甲基-1,4-二氢-3,5-吡啶二羧酸盐。无脂枣籽提取物的总酚和类黄酮含量分别为4364.704 mg /100克和17200 mg /100克。其抗氧化活性很强,对DPPH自由基的IC50值为10.1951 mg/L。抗菌活性显示金黄色葡萄球菌的抑制作用较强,而大肠杆菌的抑制作用较弱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Penentuan Profil Metabolit Sekunder, Aktivitas Antioksidan dan Antibakteri dari Ekstrak Biji Kurma (Phoenix dactylifera L.) Bebas Lipid
Dates are one of the plants that grow in the Middle East, one of them is the Golden Valley variety. Dates are usually consumed directly and can also be processed into date juice drinks. Dates refined process would produce waste in the form of date seeds are discarded and not processed into other products. This study used lipid-free Golden valley date seed extract to determine the secondary metabolite profile by using LC-MS/MS method, determining phenolic content using the Folin-Ciocalteau method, determining total flavonoids using the aluminium chloride method, determining antioxidant activity by DPPH method, and determination of antibacterial activity by the disc diffusion method. The results showed that the compounds identified from the lipid-free date seed extract were catechin-(4α→8)-catechin, bis((1S,2R,5S)-5-isopropyl-2,3-dimethylcyclo-hexyl)-2,6-dimethylpyridine-3,5-dicarboxylate, 3,5,6-trihydroxy-4',7-dimethoxyflavone, ethyl-2-amino-3-oxobutanoate, and bis(2-isopropyl-5-methylcyclohexyl)-2,6-dimethyl-1,4-dihydro-3,5-pyridine dicarboxylate. Lipid-free date seed extract contains total phenolic and flavonoid totals of 4364.704 mgGAE/100 grams of dried extract and 17200 mgQE/100 grams of dried extract. Its antioxidant activity is very strong in counteracting DPPH free radicals indicated by IC50 value of 10.1951 mg/L. Antibacterial activity shows strong inhibition of Staphylococcus aureus bacteria compared to inhibition of Escherichia coli bacteria that are categorized as weak.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
24
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信