用溶液中的动态光散射监测激活剂和抑制剂对胶原酶与胶原相互作用的影响

Q3 Physics and Astronomy

Journal of Biomedical Photonics and Engineering Pub Date : 2021-06-02 DOI:10.18287/jbpe21.07.020305

I. Sergeeva, Anna Petrova, A. Shlenskaya, G. Petrova

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引用次数: 1

摘要

使用动态光散射法监测了溶组织梭菌中细菌胶原酶对I型胶原分子的实时消化。获得了不同温度下纯“胶原蛋白+胶原酶”Tris–HCl缓冲溶液以及添加CaCl2、ZnCl2、MgCl2和EDTA的溶液的平移扩散系数（Dt）和流体动力学半径（RH）的时间依赖性。研究表明，细菌胶原酶对I型胶原分子的消化是一级反应。计算反应速率系数。

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Influence of Activators and Inhibitors on the Collagenase with Collagen Interaction Monitored by Dynamic Light Scattering in Solutions

Real–time digestion of type I collagen molecules by bacterial collagenase from Clostridium histolyticum has been monitored using Dynamic Light Scattering method. Time dependencies for translation diffusion coefficient (Dt) and hydrodynamic radius (RH) on were obtained for pure “collagen + collagenase” Tris–HCl buffer solution at different temperatures and for solutions with added CaCl2, ZnCl2 and MgCl2 and EDTA. It was shown that digestion of type I collagen molecules by bacterial collagenase is the first-order reaction. Reaction rate coefficients were calculated.

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来源期刊

Journal of Biomedical Photonics and Engineering Physics and Astronomy-Acoustics and Ultrasonics

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

8 weeks