真核起始因子4E结合蛋白家族成员在鱼类组织中广泛表达:裂腹鱼4E- bp的克隆与分布

Q1 Agricultural and Biological Sciences
Fangjun Lin , Dengyue Yuan , Defang Chen , Zhiqiong Li
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引用次数: 1

摘要

裂胸鱼(鲤形,鲤科,裂胸鱼科)是中国西南地区特有的珍贵鱼类。虽然在我们的实验室进行了一系列关于S. prenanti摄食和生长的遗传和内分泌控制的研究,但其机制尚不清楚。为了更好地了解这些机制,我们从S. prenanti中分离出真核起始因子4E结合蛋白(4E- bp1、4E- bp2和4E- bp3)的完整编码序列,并检测了它们在不同组织中的mRNA分布。S. prenanti 4E-BP1、4E-BP2和4E-BP3分别由120、113和111个氨基酸组成,与鲤科鱼类同源物具有相当高的氨基酸同源性。对4e - bp序列的系统发育分析显示,脊椎动物4e - bp有三个主要分支。然而,令人感兴趣的是硬骨鱼4E-BP1与脊椎动物4E-BP3聚集在一起,而四足动物4E-BP1形成了一个不同的分支。所有4e - bp的mrna均在大腹蛇的脑组织、垂体、眼、心、肠、肝胰腺、肾、脾、性腺、肌肉、皮肤和鳃中普遍表达。这种广泛的分布表明,4e - bp可能具有不同的生理作用,特别是对脑、垂体、肠道、肝胰腺和肌肉的作用,涉及摄食和生长调节。这一初步研究为进一步研究棘鱼和其他硬骨鱼摄食和生长调控机制开辟了道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Eukaryotic initiation factor 4E binding protein family members are widely expressed in fish tissues: Cloning and distribution of 4E-BPs in Schizothorax prenanti

Schizothorax prenanti (Cypriniformes, Cyprinidae, Schizothoracinae) is an endemic, valuable fish in the southwest of China. Although a series of studies concerning the genetic and endocrine control of feeding and growth in S. prenanti have been examined in our laboratory, the mechanism is still unclear. To better understand these mechanisms, complete coding sequences of the eukaryotic initiation factor 4E binding proteins (4E-BP1, 4E-BP2 and 4E-BP3) were isolated in S. prenanti and their mRNA distributions examined in different tissues. S. prenanti 4E-BP1, 4E-BP2 and 4E-BP3 consisted of 120, 113 and 111 amino acids and exhibited a quite high degree of amino acid identity, respectively, to their cyprinid fish homologs. Phylogenetic analysis of the 4E-BPs sequences showed three main branches of vertebrate 4E-BPs. However, it was of interest that teleost 4E-BP1 clustered with vertebrate 4E-BP3, while the tetrapod 4E-BP1 formed a distinct clade. The mRNAs of all 4E-BPs were ubiquitously expressed in S. prenanti tissues, including brain, pituitary, eye, heart, gut, hepatopancreas, kidney, spleen, gonad, muscle, skin and gill. The widespread distributions suggest that 4E-BPs might have distinct physiological actions in S. prenanti, in particular on the brain, pituitary, gut, hepatopancreas and muscle, which involved feeding and growth regulation. This preliminary study opens the way for further functional studies on the mechanisms of the regulation of feeding and growth in S. prenanti and other teleost.

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来源期刊
Agri Gene
Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
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期刊介绍: Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.
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