振动治疗可调节巨噬细胞极化并增强雌激素缺乏的骨质疏松骨折愈合中的早期炎症反应。

IF 3.2 3区 医学 Q3 CELL & TISSUE ENGINEERING
S. Chow, Y. Chim, J. Wang, N. Zhang, R. Wong, N. Tang, K. Leung, W. Cheung
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引用次数: 25

摘要

骨折愈合是一个精心安排和协调的过程,始于炎症阶段,涉及免疫细胞的浸润和细胞因子的释放,包括肿瘤坏死因子-α (TNF-α)、白细胞介素-6 (IL-6)和白细胞介素-10 (IL-10)。低震级高频振动(LMHFV)刺激能有效促进骨折愈合。本研究假设骨质疏松性骨折的先天免疫反应受损,而LMHFV可以正向调节。9月龄卵巢切除术(OVX)诱导的骨质疏松大鼠随机分为假手术组(sham)、OVX对照组(OVX)、OVX振动组(OVX- vt)或OVX振动组加COX-2特异性非甾体抗炎药组(OVX- vt - nsaid)。治疗组给予LMHFV (35 Hz, 0.3 g) 20 min/d和5 d/周。治疗后1、2、4和8周,通过每周x线片、终点微计算机断层扫描(µCT)、酶联免疫吸附测定(ELISA)和组织形态测定来评估愈合和先天免疫反应。结果显示,OVX轻微升高全身炎症反应,但局部损伤骨折部位的先天免疫反应,在愈合早期TNF-α和IL-6的表达显著降低,IL-10的表达显著升高。LMHFV通过部分恢复骨折部位受损的先天免疫反应,同时促进巨噬细胞从M1(促炎)表型向M2(抗炎)表型的极化进展,有效地加速OVX骨的延迟骨折愈合。综上所述,振动治疗可以正向调节先天免疫反应受损,促进巨噬细胞极化,促进骨质疏松骨折愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Vibration treatment modulates macrophage polarisation and enhances early inflammatory response in oestrogen-deficient osteoporotic-fracture healing.
Fracture healing is a well-orchestrated and coordinated process and begins with the inflammatory stage involving the infiltration of immune cells and the release of cytokines, including tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10). Low-magnitude high-frequency vibration (LMHFV) stimulation is effective in promoting fracture healing. The study hypothesis was that the innate immune response was impaired in osteoporotic fracture and LMHFV could positively modulate it. 9-month-old ovariectomy (OVX)-induced osteoporotic rats were randomised into sham (SHAM), OVX control (OVX), OVX-vibration (OVX-VT) or OVX vibration plus administration of COX-2 specific non-steroid anti-inflammatory drugs (OVX-VT-NSAID). LMHFV (35 Hz, 0.3 g) was given 20 min/d and 5 d/week to the treatment groups. Healing and innate immune response were evaluated by weekly radiographs, endpoint micro-computed tomography (µCT), enzyme-linked immunosorbent assay (ELISA) and histomorphometry at weeks 1, 2, 4 and 8 post-treatment. Results showed that OVX slightly elevated systemic inflammation but impaired the innate immune response locally at the fracture site, with significantly lower expressions of TNF-α and IL-6 but higher IL-10 expression during the early stage of healing. LMHFV was effective in accelerating the delayed fracture healing in OVX bones by partly restoring the impaired innate immune response at the fracture site, accompanied by promoted progression of macrophage polarisation from M1 (pro-inflammatory) to M2 (anti-inflammatory) phenotype. In conclusion, vibration treatment could positively modulate the impaired innate immune response and promote macrophage polarisation in osteoporotic-fracture healing.
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来源期刊
European cells & materials
European cells & materials 生物-材料科学:生物材料
CiteScore
6.00
自引率
6.50%
发文量
55
审稿时长
1.5 months
期刊介绍: eCM provides an interdisciplinary forum for publication of preclinical research in the musculoskeletal field (Trauma, Maxillofacial (including dental), Spine and Orthopaedics). The clinical relevance of the work must be briefly mentioned within the abstract, and in more detail in the paper. Poor abstracts which do not concisely cover the paper contents will not be sent for review. Incremental steps in research will not be entertained by eCM journal.Cross-disciplinary papers that go across our scope areas are welcomed.
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