COMPARATIVE MORPHOLOGICAL FEATURES OF SUBCUTANEOUS TISSUE OF EXPERIMENTAL ANIMALS IN RESPONSE TO THE IMPLANTATION WITH MODIFIED POLYPROPYLENE MESH ENDOPROSTHESIS

Objective. To study cellular and tissue reactions in experimental animals in response to the implantation of a polypropylene mesh endoprosthesis modified with a polycaprolactone solution by the different methods. Methods. The object of the study was modified with polypropylene mesh endoprostheses implanted in experimental animals. The animals were divided into three groups: the control group - with polypropylene mesh endoprosthesis without its modification; the first experimental group - with polycaprolactone modification by its uniform distribution on the endoprosthesis surface; the second experimental group - with the samples of polypropylene mesh endoprosthesis modified by method of electrospinning. The morphological assessment of reactions to the implantation of the materials was carried out by means of the calculation of the cellular elements of inflammatory and connective tissue origin along the periphery of the implants Results.A statistically significant increase in the number of fibroblasts was revealed on the 3rd day of observation in the animals of the second experimental group in comparison with the control one (р=0,047). The analogous trend was noted on the 7th day of the study in comparison with the control animals (р=0,002), as well as with the animals from the first experimental group (р<0,001). On the 7th day of the experiment the enhancement of the fibroplastic properties of the samples modified with polycaprolactone by electrospinning was characterized by a less pronounced inflammatory reaction of the peripheral tissues and it indicates a higher rate of granulation tissue maturation. Conclusion. Tissue and cellular reactions around the specimens modified with with polycaprolactone solution by the electrospinning method were found to be characterized by a less pronounced inflammatory reaction of the peripheral tissues and more expressed fibroplastic reaction in comparison with the control specimens. The endoprostheses obtained in this way can be used to stimulate the migration of fibroblasts and enhance local collagen synthesis, accelerate the processes of reparative regeneration in patients with undifferentiated connective tissue dysplasia. Thus it makes possible to recommend introducing this method of PSE modification in clinical practice. What this paper adds For the first time, morphological features of subcutaneous tissue around samples of polypropylene mesh endoprostheses modified with polycaprolactone solution by the different methods of its application have been studied. The changes in tissues around the samples of polypropylene mesh endoprostheses modified with a polycaprolactone solution by electrospinning are found to be characterized by a low peripheral inflammatory response of tissues with an increase of fibroplastic changes in comparison with control ones.