人类免疫缺陷病毒对循环p53、miR-21和miR-125b的调节作用:有诊断意义吗?

Jude Ogechukwu Okoye, A. Ngokere, C. C. Onyenekwe, O. Omotuyi, S. Ogenyi, C. M. Obi, S. Fasogbon
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引用次数: 1

摘要

对临床医生来说,确定有患宫颈癌风险的免疫功能低下妇女仍然是一个挑战。为了确定HIV在宫颈癌发生中的作用,本研究评估了正常下调的肿瘤因子(miR-21、miR-146a、miR-155、miR-182和miR-200c)和正常上调的肿瘤抑制因子(miR-let-7b、miR-125b、miR-143、miR-145和p53表达)在感染HIV (HIV+)和未感染HIV (HIV)的女性血清中与宫颈癌相关的水平。方法:本病例-对照研究纳入173名女性;确诊HIV+ (n = 103)和HIV - (n = 70)。采用逆转录酶PCR检测血清miRNAs和p53水平。对生成的数据进行t检验和Pearson相关分析。结果:在HIV阳性妇女中,miR-21水平高于HIV阴性妇女(p = 0.028),而在HIV阳性妇女中,miR-125和p53基因水平分别低于HIV阴性妇女(p = 0.050和0.049)。在HIV阳性妇女中,miR-21与其他肿瘤指标有显著的直接关系(p < 0.05)。结论:本研究揭示HIV通过调节miR-21、p53和miR-125b的循环水平参与宫颈癌的发生。这表明,这些生物标志物可以用来识别患宫颈癌的高风险人群。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modulatory Effect of Human Immunodeficiency Virus on Circulating p53, miR-21, and miR-125b: Any Diagnostic Implication?
Identifying immunocompromised women who are at risk of developing cervical cancer remains a challenge for clinicians. In an effort to identify the role of HIV in cervical carcinogenesis, this study evaluated the levels of normally downregulated oncomirs (miR-21, miR-146a, miR-155, miR-182, and miR-200c) and normally upregulated tumor suppressors (miR-let-7b, miR-125b, miR-143, miR-145, and p53 expression) associated with cervical cancer in the serum of women living with HIV (HIV+) and without HIV (HIV. Method: This case-control study included 173 women; confirmed HIV+ (n = 103) and HIV− (n = 70). Serum levels of miRNAs and p53 were determined using reverse transcriptase PCR. t-test and Pearson’s correlation analyses were carried out on the generated data. Result: A higher level of miR-21 was observed among HIV+ women compared with their HIV− counterpart (p = 0.028), whereas lower levels of miR-125, and p53 gene were observed among HIV+ women compared with HIV− women at p = 0.050 and 0.049, respectively. Significant direct relationships were observed between miR-21 and other oncomirs (p < 0.05) among HIV+ women. Conclusion: This study revealed that HIV contributes to cervical carcinogenesis by modulating circulating levels of miR-21, p53, and miR-125b. It suggests that these biomarkers could be used to identify at high risk for developing cervical cancer.
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