卟啉单元构成的光动力聚合物作为抗菌材料

Photochem Pub Date : 2022-11-09 DOI:10.3390/photochem2040057
M. B. Ballatore, M. E. Pérez, Sofía C. Santamarina, J. Durantini, M. Milanesio, E. Durantini
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引用次数: 0

摘要

微生物的光动力失活已成为一种有前途的策略来杀死和根除病原体。本文以5,10,15,20-四基[3-(n -乙基咔唑基)]卟啉及其配合物Zn(II)为原料,通过氧化聚合合成了两种聚合物TCP-P和ZnTCP-P。固体聚合物由直径100纳米,长度100 - 500纳米的棒状结构组成,棒状结构在表面形成微孔结构。溶液中的紫外可见吸收光谱显示出相应的结构卟啉的Soret和Q波段特征。此外,聚合物呈现两个红色发射带,TCP-P的量子产率ΦF = 0.11, ZnTCP-P的量子产率ΦF = 0.050。这些化合物敏化了单线态分子氧的生产,量子产率为ΦΔ~0.3。因此,在共轭物中卟啉单元的光谱和光动力学性质得以保持。对这两种聚合物的光动力学活性进行了灭活金黄色葡萄球菌的试验。在细胞悬液中,TCP-P比ZnTCP-P对细菌的杀伤效果更好。4µM TCP-P照射20 min后未检测到活的金黄色葡萄球菌。此外,这两种聚合物都显示出高的光毒性活性,可以根除附着在表面的金黄色葡萄球菌细胞。结果表明,这些共轭聚合物可以作为有效的抗菌剂光灭活病原体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Photodynamic Polymers Constituted by Porphyrin Units as Antibacterial Materials
Photodynamic inactivation of microorganisms has emerged as a promising strategy to kill and eradicate pathogens. In this work, two polymers, TCP-P and ZnTCP-P, were synthesized by oxidative polymerization of 5,10,15,20-tetrakis [3-(N-ethylcarbazoyl)]porphyrin and its complex with Zn(II). Solid polymers consist of rods (diameter 100 nm, length ~100–500 nm) that form microporous structures on a surface. UV-visible absorption spectra in solution showed the Soret and Q bands characteristic of the corresponding constitutional porphyrins. In addition, the polymers presented two red emission bands with quantum yields ΦF = 0.11 for TCP-P and ΦF = 0.050 for ZnTCP-P. These compounds sensitized the production of singlet molecular oxygen with quantum yields of ΦΔ~0.3. Thus, the spectroscopic and photodynamic properties of the porphyrin units were maintained in the conjugates. The photodynamic activity induced by both polymers was tested to inactivate S. aureus. In cell suspensions, TCP-P was more effective than ZnTCP-P in killing bacteria. Viable S. aureus cells were not detected using 4 µM TCP-P after 20 min of irradiation. Moreover, both polymers showed a high photocytotoxic activity to eradicate S. aureus cells attached to a surface. The results indicate that these conjugated polymers can act as effective antimicrobial agents to photoinactivate pathogens.
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CiteScore
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