伊朗Sanandaj草莓样品中大肠杆菌TEM和SHV β -内酰胺酶基因的研究

Q3 Agricultural and Biological Sciences

Journal of Food Quality and Hazards Control Pub Date : 2021-09-10 DOI:10.18502/jfqhc.8.3.7199

M. Dallal, H. Abdolmaleki, R. Ramazanzadeh, R. M. Fard

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引用次数: 0

摘要

背景:当使用动物粪便时，食品中可能含有致病菌，尤其是大肠杆菌。本研究的主要目的是研究从伊朗Sanandaj草莓样品中分离的大肠杆菌中的TEM (blaTEM)和SHV β -内酰胺酶(blaSHV)基因。方法:在本研究中，从伊朗Sanandaj的农场(传统)、温室和包装中收集了150个草莓样品。大肠杆菌污染采用常规培养方法。然后，利用表型和基因型方法分别对分离株进行了广谱β -内酰胺酶(ESBL)产生和blaTEM或blaSHV基因的研究。结果:大肠杆菌对抗菌药物的敏感性和耐药性分别与氯霉素和甲氧苄啶/磺胺甲恶唑有关。21株大肠杆菌中，8株对头孢他啶和头孢噻肟耐药;其中6株为esbl产生菌。此外，对6株产ESBL的大肠杆菌进行聚合酶链反应(PCR)分析发现，4株分离株含有blaTEM基因，未分离株含有blaSHV基因。结论:在本研究中，大肠杆菌分离株中发现了多种抗生素耐药模式，特别是从伊朗生产的草莓中分离的大肠杆菌中发现了ESBL模式。

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Investigation of TEM and SHV Beta-Lactamase Genes in Escherichia coli Isolated from Strawberry Samples in Sanandaj, Iran

Background: When animal manures are used, food products may include pathogenic bacteria, especially Escherichia coli. The major aim of the current study was to investigate TEM (blaTEM) and SHV beta-lactamase (blaSHV) genes in E. coli isolated from strawberry samples in Sanandaj, Iran. Methods: In this study, 150 strawberry samples were collected from farms (traditional), greenhouses, and packages in Sanandaj, Iran. E. coli contamination was done using routine culture methods. Then, isolates were investigated for Extended-Spectrum Beta-Lactamase (ESBL) production and blaTEM or blaSHV genes using phenotypic and genotypic methods, respectively. Results: The most susceptibility and resistance of E. coli to antibiotics were related to chloramphenicol and trimethoprim/sulfamethoxazole, respectively. Out of 21 isolates of E. coli, eight were resistant to ceftazidime and cefotaxime; from which, six isolates were ESBL-producer. Furthermore, Polymerase Chain Reaction (PCR) analysis of six ESBL- producing E. coli isolates showed that four isolates included blaTEM gene, while no isolates included blaSHV gene. Conclusion: In this study, multiple antibiotic resistance patterns were seen in E. coli isolates, especially ESBL patterns in E. coli isolated from strawberries produced in Iran.

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来源期刊

Journal of Food Quality and Hazards Control Agricultural and Biological Sciences-Food Science

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

8 weeks

期刊介绍： Journal of Food Quality and Hazards Control (J. Food Qual. Hazards Control) is an international peer-reviewed quarterly journal that aims at publishing of high quality articles involved in food quality, food hygiene, food safety, and food control which scientists from all over the world may submit their manuscript. This academic journal aims to improve international exchange of new findings and recent developments in all aspects of agricultural and biological sciences. This free of charge journal is published in both online and print forms and welcomes the manuscripts that fulfill the general criteria of novelty and scientific importance. Among the most significant objectives of Journal of Food Quality and Hazards Control are to ensure that the articles reflect a wide range of topics regarding journal scopes; to do a fair, scientific, fast, as well as high quality peer-review process; to provide a wide and diverse geographical coverage of articles around the world; and to publish the articles having a trustable resource of scientific information for the audiences. The types of acceptable submissions include original article, review article, short communication, letter to the editor, case report, editorial, as well as book review. Journal of Food Quality and Hazards Control is an official journal of Research Center for Food Hygiene and Safety, School of Public Health, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.