低温大气等离子体通过ros介导的PI3K/Akt/mTOR信号通路抑制CAL-62细胞的增殖

IF 1 4区工程技术 Q3 NUCLEAR SCIENCE & TECHNOLOGY

Science and Technology of Nuclear Installations Pub Date : 2022-06-08 DOI:10.1155/2022/3884695

Fang Liu, Yuanyuan Zhou, Wencheng Song, Hongzhi Wang

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引用次数: 2

摘要

本研究旨在探讨冷大气等离子体(CAP)对间变性甲状腺癌细胞(CAL-62细胞)的抑制作用及其分子机制。通过细胞活力、超氧化物歧化酶活性、细胞凋亡、细胞周期和蛋白表达水平评估CAP对CAL-62细胞的影响，并探讨血浆产生的活性氧(ROS)的作用。结果表明，CAP通过增加细胞ROS水平，呈剂量依赖性地抑制细胞活力，促进细胞凋亡和G2/M阻滞。CAP可增强细胞超氧化物歧化酶(SOD)活性，表明细胞的抗氧化系统被激活。另外，CAP产生的ROS可以通过抑制PI3K/Akt/mTOR信号通路抑制CAL-62细胞的增殖。本研究结果将为CAP在甲状腺间变性癌治疗中的应用提供有益的支持。

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Cold Atmospheric Plasma Inhibits the Proliferation of CAL-62 Cells through the ROS-Mediated PI3K/Akt/mTOR Signaling Pathway

This study aimed to investigate the inhibitory effects of cold atmospheric plasma (CAP) on anaplastic thyroid cancer cells (CAL-62 cells) and to reveal the molecular mechanism. The effects of CAP on CAL-62 cells were evaluated by cell viability, superoxide dismutase activity, apoptosis, cell cycle, and protein expression level, and the role of reactive oxygen species (ROS) produced by plasma was also investigated. The results showed that CAP dose-dependently inhibited cell viability and promotes cell apoptosis and G2/M arrest by increasing cell ROS levels. The activity of superoxide dismutase (SOD) was enhanced by CAP which indicated that the antioxidant system of the cell was activated. Additionally, the ROS produced by CAP can inhibit CAL-62 cell proliferation by inhibiting the PI3K/Akt/mTOR signaling pathway. Therefore, these findings will provide useful support for the application of CAP for treating anaplastic thyroid cancer.

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来源期刊

Science and Technology of Nuclear Installations NUCLEAR SCIENCE & TECHNOLOGY-

CiteScore

2.30

自引率

9.10%

发文量

审稿时长

4-8 weeks

期刊介绍： Science and Technology of Nuclear Installations is an international scientific journal that aims to make available knowledge on issues related to the nuclear industry and to promote development in the area of nuclear sciences and technologies. The endeavor associated with the establishment and the growth of the journal is expected to lend support to the renaissance of nuclear technology in the world and especially in those countries where nuclear programs have not yet been developed.