金丝桃苷通过NF-κB/PTEN/JAK2通路促进胃癌细胞凋亡及抑制细胞活力

Pub Date : 2022-06-15 DOI:10.31901/24566330.2022/22.03.819

Bo Shu

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引用次数: 0

摘要

本研究探讨金丝桃苷对胃癌细胞活力和凋亡的影响。采用CCK-8和流式细胞术检测HGC-27细胞活力和凋亡，结果表明金丝桃苷处理抑制了细胞活力，但促进了细胞凋亡。RT-qPCR结果显示，金丝桃苷处理后，HGC-27细胞中Bcl-2 mRNA表达下调，Bax和caspase-3 mRNA表达升高。此外，ELISA检测了金丝桃苷处理后HGC-27细胞NF-κB/PTEN/JAK信号通路蛋白浓度的变化。结果表明，金丝桃苷处理可降低磷酸化NF-κB/ p65蛋白浓度，提高PTEN和JAK2蛋白浓度。

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Hyperoside Facilitates Gastric Cancer (GC) Cell Apoptosis and Inhibits Viability via NF-κB/PTEN/JAK2 Pathway

This study investigated impacts of hyperoside on viabilities and apoptosis of gastric cancer (GC) cells. CCK-8 and flow cytometry were applied for examining HGC-27 cell viabilities and apoptosis, indicating that hyperoside treatment suppressed cell viabilities but facilitated cell apoptosis. Additionally, RT-qPCR results revealed that mRNA expressions of Bcl-2 in HGC-27 cells were downregulated while Bax and caspase-3 were elevated with hyperoside treatment. Furthermore, ELISA examined changes about protein concentrations of NF-κB/PTEN/JAK signalling pathway in HGC-27 cells after being treated by hyperoside. Results showed that protein concentrations of phosphorylated NF-κB/ p65 were reduced with hyperoside treatment while PTEN and JAK2 protein concentrations were promoted.

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