三级保健医院念珠菌分离株的临床菌学研究:一项初步研究

Q4 Medicine
S. Mishra, A. Subramanian, A. Kindo
{"title":"三级保健医院念珠菌分离株的临床菌学研究:一项初步研究","authors":"S. Mishra, A. Subramanian, A. Kindo","doi":"10.4103/jms.jms_28_21","DOIUrl":null,"url":null,"abstract":"Background: Candida infection is on the rise with an increasing number of nonalbicans species. Therefore, the need to speciate Candida rapidly and accurately is of the utmost importance. The purpose of this study was to speciate Candida using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), to analyze the correlation of the isolates with the clinical condition, and to study the outcome of the patient. Materials and Methods: PCR-RFLP using universal primers ITS1 and ITS4 was done to speciate all isolates of Candida; patient details were collected to analyze the clinical condition and the outcome of the patient. Results: The most common species of Candida isolated was Candida tropicalis 14 (56%) followed by Candida albicans 5 (20%), Candida auris 3 (14%), Candida parapsilosis 1 (4%), Candida orthopsilosis 1 (4%), and Candida kefyr 1 (4%). Majority of the samples that were collected were urine samples 15 (60%). The average duration of hospital stay was found to be 13.8 days. A number of underlying risk factors were present such as patients with diabetes, sepsis, malignancy, covid19 infection, surgical patients, preterm patients, elderly patients, and patients on long-term steroids. Conclusion: Candidemia is on the rise nowadays with nonalbicans species responsible for the majority of the infections. Since the outcome of the patient depends on rapid diagnosis and prompt initiation of antifungal agents PCR-RFLP proves to be a rapid and reliable test to identify most of the prevailing species of Candida.","PeriodicalId":39636,"journal":{"name":"JMS - Journal of Medical Society","volume":"35 1","pages":"58 - 62"},"PeriodicalIF":0.0000,"publicationDate":"2021-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Clinicomycological study of Candida isolates in a tertiary care hospital: A pilot study\",\"authors\":\"S. Mishra, A. Subramanian, A. Kindo\",\"doi\":\"10.4103/jms.jms_28_21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Candida infection is on the rise with an increasing number of nonalbicans species. Therefore, the need to speciate Candida rapidly and accurately is of the utmost importance. The purpose of this study was to speciate Candida using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), to analyze the correlation of the isolates with the clinical condition, and to study the outcome of the patient. Materials and Methods: PCR-RFLP using universal primers ITS1 and ITS4 was done to speciate all isolates of Candida; patient details were collected to analyze the clinical condition and the outcome of the patient. Results: The most common species of Candida isolated was Candida tropicalis 14 (56%) followed by Candida albicans 5 (20%), Candida auris 3 (14%), Candida parapsilosis 1 (4%), Candida orthopsilosis 1 (4%), and Candida kefyr 1 (4%). Majority of the samples that were collected were urine samples 15 (60%). The average duration of hospital stay was found to be 13.8 days. A number of underlying risk factors were present such as patients with diabetes, sepsis, malignancy, covid19 infection, surgical patients, preterm patients, elderly patients, and patients on long-term steroids. Conclusion: Candidemia is on the rise nowadays with nonalbicans species responsible for the majority of the infections. Since the outcome of the patient depends on rapid diagnosis and prompt initiation of antifungal agents PCR-RFLP proves to be a rapid and reliable test to identify most of the prevailing species of Candida.\",\"PeriodicalId\":39636,\"journal\":{\"name\":\"JMS - Journal of Medical Society\",\"volume\":\"35 1\",\"pages\":\"58 - 62\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"JMS - Journal of Medical Society\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jms.jms_28_21\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"JMS - Journal of Medical Society","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jms.jms_28_21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

摘要

背景:念珠菌感染呈上升趋势,非念珠菌的种类越来越多。因此,快速准确地对念珠菌进行物种鉴定是至关重要的。本研究的目的是利用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)分离念珠菌,分析分离株与临床状况的相关性,并研究患者的预后。材料与方法:应用通用引物ITS1和ITS4进行PCR-RFLP,对所有念珠菌分离株进行特异性鉴定;收集患者详细信息以分析患者的临床状况和结果。结果:分离出的假丝酵母最常见的种类为热带假丝酵母14种(56%),其次为白色假丝酵母5种(20%)、耳假丝酵母3种(14%)、近裸假丝酵母1种(4%)、正丝假丝酵母(4%)和kefyr假丝酵母菌1种(4%)。收集到的大多数样本是15份尿液样本(60%)。平均住院时间为13.8天。存在许多潜在的风险因素,如糖尿病、败血症、恶性肿瘤、新型冠状病毒感染患者、外科患者、早产患者、老年患者和长期服用类固醇的患者。结论:Candidemia目前呈上升趋势,大多数感染是由非感染者引起的。由于患者的预后取决于快速诊断和及时开始使用抗真菌药物,PCR-RFLP被证明是一种快速可靠的检测方法,可以识别大多数念珠菌的主要种类。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Clinicomycological study of Candida isolates in a tertiary care hospital: A pilot study
Background: Candida infection is on the rise with an increasing number of nonalbicans species. Therefore, the need to speciate Candida rapidly and accurately is of the utmost importance. The purpose of this study was to speciate Candida using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), to analyze the correlation of the isolates with the clinical condition, and to study the outcome of the patient. Materials and Methods: PCR-RFLP using universal primers ITS1 and ITS4 was done to speciate all isolates of Candida; patient details were collected to analyze the clinical condition and the outcome of the patient. Results: The most common species of Candida isolated was Candida tropicalis 14 (56%) followed by Candida albicans 5 (20%), Candida auris 3 (14%), Candida parapsilosis 1 (4%), Candida orthopsilosis 1 (4%), and Candida kefyr 1 (4%). Majority of the samples that were collected were urine samples 15 (60%). The average duration of hospital stay was found to be 13.8 days. A number of underlying risk factors were present such as patients with diabetes, sepsis, malignancy, covid19 infection, surgical patients, preterm patients, elderly patients, and patients on long-term steroids. Conclusion: Candidemia is on the rise nowadays with nonalbicans species responsible for the majority of the infections. Since the outcome of the patient depends on rapid diagnosis and prompt initiation of antifungal agents PCR-RFLP proves to be a rapid and reliable test to identify most of the prevailing species of Candida.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
JMS - Journal of Medical Society
JMS - Journal of Medical Society Medicine-Medicine (all)
CiteScore
0.20
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信