Cheilocostus specious（J.Koenig）C.D.Specht（Costaceae）根状茎的mokko内酯对暴发性肝衰竭的保护作用

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.55730/1300-008x.2764

A. Altyar, Ikhlas Sindi, Dina S. El-Agamy, Wael M Elsaed, Ahmed M. Shehata, Hossam M. Abdallah, G. Mohamed, Abdelsattar M. Omar, Sabrin R. M. Ibrahim

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引用次数: 0

摘要

:香姜(英文:Cheilocostus speciosus)是Costaceae科的一种，被广泛应用于各种传统药物中，用于治疗各种疾病。木瓜内酯(ML)属倍半萜类植物愈创木酚内酯家族，从木瓜中分离得到。具有明显的抗炎和抗氧化能力。本研究探讨了ML对小鼠FHF(暴发性肝衰竭)的肝保护潜力。以雄性瑞士白化病小鼠为模型，腹腔注射半乳糖胺(γ - γ， 700 mg/kg)/脂多糖(LPS, 10µg/kg)，成功建立FHF。小鼠每天口服ML(20或40 mg/kg，口服)预处理5天，然后再给药。注射D-GalN/LPS 8小时后，采集血清和肝组织进行不同的生化、组织学分级、免疫组织化学和ELISA分析。结果表明，d-GalN/ lps诱导了大量肝脏损伤，血清生化参数:转氨酶、γ-GT和ALP显著升高，肝脏组织病理结构恶化。d-GalN/LPS在肝细胞中造成氧化损伤状态，因为MDA水平显著升高，同时抗氧化剂如GSH(还原谷胱甘肽)、SOD(超氧化物歧化酶)活性和TAC(总抗氧化能力)下降。此外，d-GalN/LPS激活NF-κB(核因子κ b)，导致TNF-α、IL-6和IL-1β的产生增加。有趣的是，ML预处理后所有这些变化都得到了改善，表明ML对d-GalN/ lps诱导的FHF具有有效的肝保护、抗氧化和抗炎作用。这些作用可能是通过抑制NF-κB/下游细胞因子信号传导介导的。因此，ML被认为是治疗FHF的新候选药物。

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Protective potential of mokko lactone from Cheilocostus speciosus (J. Koenig) C.D.Specht (Costaceae) rhizomes against fulminant hepatic failure

: Cheilocostus speciosus (Syn : Costus speciosus ) (Crepe or spiral ginger) is one of the species of the family Costaceae that is widely used in various traditional medicines for treating various ailments. Mokko lactone (ML) belongs to the guaianolide family of sesquiterpenes, which has been separated from C. speciosus . It showed noticeable antiinflammation and antioxidative capacities. The current study explored the ML hepatoprotective potential against FHF (fulminant hepatic failure) in mice. FHF was successfully established using intraperitoneal injection of ᴅ -galactosamine ( ᴅ -GalN, 700 mg/kg)/lipopolysaccharide (LPS, 10 µg/kg) in male Swiss-albino mice. The mice were pretreated with ML (20 or 40 mg/kg, orally) daily for five days before ᴅ -GalN/LPS challenge. Eight hours after D-GalN/LPS injection, serum and hepatic tissue were harvested for different biochemical, histologic grading, immunohistochemical, and ELISA analyses. The results have shown that d-GalN/LPS-induced massive hepatic damage that was evident through the tremendous increase in serum biochemical parameters: transaminases, γ-GT, and ALP, as well as deteriorated histopathological architecture of the liver. d-GalN/LPS created a state of oxidative damage in the hepatocytes as there was a significant rise in MDA level concurrently with depressed antioxidants as GSH (reduced glutathione), SOD (superoxide dismutase) activity, and TAC (total antioxidant capacity). Ad-ditionally, d-GalN/LPS activated NF-κB (nuclear factor kappa-B), resulting in an increase in the production of TNF-α, IL-6, and IL-1β. Interestingly, all these changes were amended by ML pretreatment, indicating the potent hepatoprotective, antioxidant, and antiinflammatory efficacy of ML against d-GalN/LPS-induced FHF. These effects could be mediated through inhibition of NF-κB/downstream cytokine signaling. Hence, ML is suggested as new candidate for the treatment of FHF.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.