{"title":"苏丹尼罗河五种重要淡水鱼的DNA条形码","authors":"A. Hamza, A. T. M. Mohammed","doi":"10.2989/16085914.2023.2236646","DOIUrl":null,"url":null,"abstract":"The study was conducted to barcode five freshwater fish species obtained from Nile River tributaries (the Atbara River and the Blue Nile), in March 2019, using analysis of partial CO1 gene sequences. Fish samples were identified morphologically as Nile perch Lates niloticus, Nile labeo Labeo vulgaris, North African catfish Clarias gariepinus, Cornish jack Mormyrops anguilloides and bebe mormyrid Hyperopisus bebe. Following DNA extraction, universal primers were used for amplification of the CO1 barcode region. The PCR products were sequenced and used for molecular identification. Each sequence was matched to corresponding GenBank sequences using the BLAST technique. Sequences of all specimens were aligned along with the GenBank published sequences and polymorphic sites were identified. The analysed sequences were uploaded to GenBank under the accession numbers OL804282– OL804286. The analysed partial CO1 barcode gene showed a readable length of ∼548–669 base pairs. Sequence comparison of CO1 gene sequences with sequences published in GenBank revealed 99.27–100% identities at the species level. Therefore, the CO1 barcodes accurately identified all the studied freshwater fishes. This is the first molecular DNA barcode characterisation of freshwater fish species found in Sudan. The fish CO1 gene sequences revealed in this study will serve as barcode references for future characterisations of Nile River fishes.","PeriodicalId":7864,"journal":{"name":"African Journal of Aquatic Science","volume":"48 1","pages":"338 - 343"},"PeriodicalIF":1.1000,"publicationDate":"2023-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"DNA barcoding of five economically important freshwater fish species from the Nile River, Sudan\",\"authors\":\"A. Hamza, A. T. M. Mohammed\",\"doi\":\"10.2989/16085914.2023.2236646\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The study was conducted to barcode five freshwater fish species obtained from Nile River tributaries (the Atbara River and the Blue Nile), in March 2019, using analysis of partial CO1 gene sequences. Fish samples were identified morphologically as Nile perch Lates niloticus, Nile labeo Labeo vulgaris, North African catfish Clarias gariepinus, Cornish jack Mormyrops anguilloides and bebe mormyrid Hyperopisus bebe. Following DNA extraction, universal primers were used for amplification of the CO1 barcode region. The PCR products were sequenced and used for molecular identification. Each sequence was matched to corresponding GenBank sequences using the BLAST technique. Sequences of all specimens were aligned along with the GenBank published sequences and polymorphic sites were identified. The analysed sequences were uploaded to GenBank under the accession numbers OL804282– OL804286. The analysed partial CO1 barcode gene showed a readable length of ∼548–669 base pairs. Sequence comparison of CO1 gene sequences with sequences published in GenBank revealed 99.27–100% identities at the species level. Therefore, the CO1 barcodes accurately identified all the studied freshwater fishes. This is the first molecular DNA barcode characterisation of freshwater fish species found in Sudan. The fish CO1 gene sequences revealed in this study will serve as barcode references for future characterisations of Nile River fishes.\",\"PeriodicalId\":7864,\"journal\":{\"name\":\"African Journal of Aquatic Science\",\"volume\":\"48 1\",\"pages\":\"338 - 343\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2023-07-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"African Journal of Aquatic Science\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.2989/16085914.2023.2236646\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MARINE & FRESHWATER BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"African Journal of Aquatic Science","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.2989/16085914.2023.2236646","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
DNA barcoding of five economically important freshwater fish species from the Nile River, Sudan
The study was conducted to barcode five freshwater fish species obtained from Nile River tributaries (the Atbara River and the Blue Nile), in March 2019, using analysis of partial CO1 gene sequences. Fish samples were identified morphologically as Nile perch Lates niloticus, Nile labeo Labeo vulgaris, North African catfish Clarias gariepinus, Cornish jack Mormyrops anguilloides and bebe mormyrid Hyperopisus bebe. Following DNA extraction, universal primers were used for amplification of the CO1 barcode region. The PCR products were sequenced and used for molecular identification. Each sequence was matched to corresponding GenBank sequences using the BLAST technique. Sequences of all specimens were aligned along with the GenBank published sequences and polymorphic sites were identified. The analysed sequences were uploaded to GenBank under the accession numbers OL804282– OL804286. The analysed partial CO1 barcode gene showed a readable length of ∼548–669 base pairs. Sequence comparison of CO1 gene sequences with sequences published in GenBank revealed 99.27–100% identities at the species level. Therefore, the CO1 barcodes accurately identified all the studied freshwater fishes. This is the first molecular DNA barcode characterisation of freshwater fish species found in Sudan. The fish CO1 gene sequences revealed in this study will serve as barcode references for future characterisations of Nile River fishes.
期刊介绍:
The African Journal of Aquatic Science is an international journal devoted to the study of the aquatic sciences, covering all African inland and estuarine waters. The Journal publishes peer-reviewed original scientific papers and short articles in all the aquatic science fields including limnology, hydrobiology, ecology, conservation, biomonitoring, management, water quality, ecotoxicology, biological interactions, physical properties and human impacts on African aquatic systems.