种猪群每周猪繁殖和呼吸综合征病毒暴露与随后的病毒脱落和苗圃死亡率之间的关系

IF 0.7 4区 农林科学 Q3 Agricultural and Biological Sciences
G. Trevisan, R. Robbins, J. Angulo, L. Dufresne, W. Lopez, Nubia R. Macedo, D. Linhares
{"title":"种猪群每周猪繁殖和呼吸综合征病毒暴露与随后的病毒脱落和苗圃死亡率之间的关系","authors":"G. Trevisan, R. Robbins, J. Angulo, L. Dufresne, W. Lopez, Nubia R. Macedo, D. Linhares","doi":"10.54846/jshap/1177","DOIUrl":null,"url":null,"abstract":"Objective: Describe the relationship of weekly breeding herd status based on processing fluid (PF) testing for porcine reproductive and respiratory syndrome virus (PRRSV) RNA by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) on subsequent viral shedding and cumulative mortality during the nursery phase. Materials and methods: Weekly cohorts (n = 121) of newborn piglets were classified into PRRSV exposure groups according to PRRSV detection in PF: low (quantification cycles [Cq] ≤ 27), medium (27 < Cq ≤ 34), high (34 < Cq ≤ 37), and negative (Cq > 37). At 6 weeks of age, oral fluids (OF) were collected from a subset of 41 cohorts, tested by qRT-PCR, and results used to classify the nursery shedding status into the same aforementioned categories. Cumulative nursery mortality was recorded for all 121 cohorts and compared between the different PRRSV exposure groups. Test agreement was assessed between PF and OF results of 41 cohorts. Moreover, the effect of 4:1 OF pooling on the probability of testing qRT-PCR-positive was evaluated. Results: The nursery mortality for low Cq cohorts was 3.40 percentage points (range, 3.28-3.99) higher than other exposure groups. Overall, Cq values were higher in PF than in OF samples, and fair agreement (κ = 0.2398) between PF and OF was encountered. Compared to individual samples, 4:1 OF pooling resulted in 100% specificity and 76.92% sensitivity. Implications: Weekly PF testing for PRRSV allowed for exposure group classification for each pig batch produced, which was a good predictor of subsequent cumulative nursery mortality.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":" ","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Relationship between weekly porcine reproductive and respiratory syndrome virus exposure in breeding herds and subsequent viral shedding and mortality in the nursery\",\"authors\":\"G. Trevisan, R. Robbins, J. Angulo, L. Dufresne, W. Lopez, Nubia R. Macedo, D. Linhares\",\"doi\":\"10.54846/jshap/1177\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: Describe the relationship of weekly breeding herd status based on processing fluid (PF) testing for porcine reproductive and respiratory syndrome virus (PRRSV) RNA by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) on subsequent viral shedding and cumulative mortality during the nursery phase. Materials and methods: Weekly cohorts (n = 121) of newborn piglets were classified into PRRSV exposure groups according to PRRSV detection in PF: low (quantification cycles [Cq] ≤ 27), medium (27 < Cq ≤ 34), high (34 < Cq ≤ 37), and negative (Cq > 37). At 6 weeks of age, oral fluids (OF) were collected from a subset of 41 cohorts, tested by qRT-PCR, and results used to classify the nursery shedding status into the same aforementioned categories. Cumulative nursery mortality was recorded for all 121 cohorts and compared between the different PRRSV exposure groups. Test agreement was assessed between PF and OF results of 41 cohorts. Moreover, the effect of 4:1 OF pooling on the probability of testing qRT-PCR-positive was evaluated. Results: The nursery mortality for low Cq cohorts was 3.40 percentage points (range, 3.28-3.99) higher than other exposure groups. Overall, Cq values were higher in PF than in OF samples, and fair agreement (κ = 0.2398) between PF and OF was encountered. Compared to individual samples, 4:1 OF pooling resulted in 100% specificity and 76.92% sensitivity. Implications: Weekly PF testing for PRRSV allowed for exposure group classification for each pig batch produced, which was a good predictor of subsequent cumulative nursery mortality.\",\"PeriodicalId\":17095,\"journal\":{\"name\":\"Journal of Swine Health and Production\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2020-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Swine Health and Production\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.54846/jshap/1177\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Swine Health and Production","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.54846/jshap/1177","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 4

摘要

目的:通过定量逆转录聚合酶链反应(qRT-PCR)对猪繁殖与呼吸综合征病毒(PRRSV)RNA进行处理液(PF)检测,描述每周繁殖群状态与随后的病毒脱落和育婴期累积死亡率的关系。材料和方法:根据PF中PRRSV的检测,将新生仔猪的每周队列(n=121)分为PRRSV暴露组:低(定量周期[Cq]≤27)、中(2737)。在6周大时,从41个队列的子集中收集口腔液(of),通过qRT-PCR进行测试,结果用于将托儿所脱落状态分类为上述相同类别。记录了所有121个队列的累计托儿所死亡率,并在不同PRRSV暴露组之间进行了比较。对41个队列的PF和OF结果之间的测试一致性进行了评估。此外,评估了4:1 of合并对检测qRT-PCR阳性概率的影响。结果:低Cq人群的托儿所死亡率比其他暴露组高3.40个百分点(范围3.28-3.99)。总体而言,PF中的Cq值高于OF样本,PF和OF之间的一致性(κ=0.2398)尚可。与单个样本相比,4:1的OF合并产生了100%的特异性和76.92%的敏感性。影响:每周对PRRSV进行PF检测,可以对每批生产的猪进行暴露组分类,这是后续累计育婴死亡率的良好预测指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Relationship between weekly porcine reproductive and respiratory syndrome virus exposure in breeding herds and subsequent viral shedding and mortality in the nursery
Objective: Describe the relationship of weekly breeding herd status based on processing fluid (PF) testing for porcine reproductive and respiratory syndrome virus (PRRSV) RNA by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) on subsequent viral shedding and cumulative mortality during the nursery phase. Materials and methods: Weekly cohorts (n = 121) of newborn piglets were classified into PRRSV exposure groups according to PRRSV detection in PF: low (quantification cycles [Cq] ≤ 27), medium (27 < Cq ≤ 34), high (34 < Cq ≤ 37), and negative (Cq > 37). At 6 weeks of age, oral fluids (OF) were collected from a subset of 41 cohorts, tested by qRT-PCR, and results used to classify the nursery shedding status into the same aforementioned categories. Cumulative nursery mortality was recorded for all 121 cohorts and compared between the different PRRSV exposure groups. Test agreement was assessed between PF and OF results of 41 cohorts. Moreover, the effect of 4:1 OF pooling on the probability of testing qRT-PCR-positive was evaluated. Results: The nursery mortality for low Cq cohorts was 3.40 percentage points (range, 3.28-3.99) higher than other exposure groups. Overall, Cq values were higher in PF than in OF samples, and fair agreement (κ = 0.2398) between PF and OF was encountered. Compared to individual samples, 4:1 OF pooling resulted in 100% specificity and 76.92% sensitivity. Implications: Weekly PF testing for PRRSV allowed for exposure group classification for each pig batch produced, which was a good predictor of subsequent cumulative nursery mortality.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
1.80
自引率
0.00%
发文量
29
审稿时长
>36 weeks
期刊介绍: The Journal of Swine Health & Production (JSHAP) is an open-access and peer-reviewed journal published by the American Association of Swine Veterinarians (AASV) since 1993. The aim of the journal is the timely publication of peer-reviewed papers with a scope that encompasses the many domains of applied swine health and production, including the diagnosis, treatment, management, prevention and eradication of swine diseases, welfare & behavior, nutrition, public health, epidemiology, food safety, biosecurity, pharmaceuticals, antimicrobial use and resistance, reproduction, growth, systems flow, economics, and facility design. The journal provides a platform for researchers, veterinary practitioners, academics, and students to share their work with an international audience. The journal publishes information that contains an applied and practical focus and presents scientific information that is accessible to the busy veterinary practitioner as well as to the research and academic community. Hence, manuscripts with an applied focus are considered for publication, and the journal publishes original research, brief communications, case reports/series, literature reviews, commentaries, diagnostic notes, production tools, and practice tips. All manuscripts submitted to the Journal of Swine Health & Production are peer-reviewed.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信