植物和部分非脊椎动物属中5S nrDNA单元类的鉴定及其在物种关系研究中的潜力

B. Baum, Douglas A. Johnson
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引用次数: 0

摘要

我们已经研究了5snrDNA的效用,这是一个保守的、多拷贝的基因家族,被组织成串联重复序列,作为各种属的遗传分析工具。先前在小麦科(Poacee)的工作表明,基于5snrDNA NTS的单位类别的先验识别极大地促进了分析。我们研究了在其他植物属和几个动物属中定义单位类的潜力,作为未来系统发育分析的一步。我们的研究结果表明,在几个动植物属中,GenBank中有足够数量的DNA材料™ 指出物种内不同的单位类,在一些属中,在物种之间发现了几个潜在的单位类。这些结果证明了物种内更深入的采样和物种内更广泛的采样是合理的。对额外DNA序列的进一步研究将有助于定义真正的单位类别。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Identification 5S nrDNA Unit Classes in Genera of Plants and Selected Non-Vertebrate Animals and Their Potential for the Study of Species Relationships
We have investigated the utility of the 5S nrDNA, a conserved, multicopy gene family organized into tandem repeats as a tool for genetic analyses in a wide variety of genera. Previous work in the Triticeae (Poaceae) demonstrated that the prior identification of unit classes based upon the 5S nrDNA NTS, greatly facilitates analysis. We investigated the potential of defining unit classes in other plant genera and several animal genera as a step towards future phylogenetic analyses. Our results demonstrate that in in several plant and animal genera there are sufficient numbers of DNA accessions in GenBank™ to point to different unit classes within species and that in some genera several potential unit classes are found across species. These results justify both more in-depth sampling within species and more breadth in sampling within. Further investigation of additional DNA sequences will help to define true unit classes.
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