具有抗氧化活性的牛胶原水解产物的制备响应面法优化

IF 2.3 Q3 PHARMACOLOGY & PHARMACY
B. Pakbin, Samaneh Allahyari, Shaghayegh Pishkhan Dibazar, W. Brück, R. Vahidi, R. Mahmoudi, A. Khanjari
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引用次数: 1

摘要

抗氧化剂广泛应用于制药工业。明胶是肉类工业的副产品,其水解产物显示出多种功能,如抗氧化活性。本研究的目的是描述和优化酶水解条件,包括时间、温度、pH和酶/底物比(E/S),通过RSM从牛明胶中制备具有抗氧化功能的蛋白质水解产物;使用DPPH方法评价清除活性。观察到的模型具有理想的充分性和充分性。我们发现,抗氧化活性随着pH值、E/S比和酶处理时间的增加而显著增加(p<0.05);但是,温度对水解产物的抗氧化活性没有显著影响(p<0.05)。胰蛋白酶水解2小时后,观察到最佳水解条件为温度35.3°C,pH 8.0,E/S比为2.5。结果表明,在这些条件下,经RSM优化的水解产物具有更高的抗氧化活性。就抗氧化潜力而言,明胶水解物可在制药工业中用作抗氧化剂补充剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Production of Bovine Collagen Hydrolysate with Antioxidant Activity; Optimized by Response Surface Methodology
Antioxidants are widely used in pharmaceutical industries. Gelatin is a byproduct of the meat industry and its hydrolysates showed several functionalities, such as antioxidant activity. The purpose of this study was to describe and optimize the enzymatic hydrolysis conditions including time, temperature, pH, and enzyme/substrate ratio (E/S) to produce protein hydrolysate with antioxidant functionality from bovine gelatin by RSM; the scavenging activity was evaluated using the DPPH method. The model observed was fitted with desirable adequacy and sufficiency. We found that the antioxidant activity increased significantly (p < 0.05) with the increase in pH value, E/S ratio, and time of enzymatic process; however, the temperature had no significant (p < 0.05) effect on the antioxidant activity of the hydrolysate. The optimum hydrolysis conditions were observed at a temperature of 35.3 °C, pH of 8.0, and E/S ratio at 2.5 after 2 h hydrolysis by trypsin enzyme. The results showed that the hydrolysate under these conditions, optimized by RSM, could be more effective on antioxidant activity. Regarding the antioxidant potential, gelatin hydrolysate can be used as an antioxidant supplement in pharmaceutical industries.
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来源期刊
Scientia Pharmaceutica
Scientia Pharmaceutica Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
4.60
自引率
4.00%
发文量
67
审稿时长
10 weeks
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