Proteus sp.通过胆碱代谢途径对三甲胺N-氧化物产生的影响以及印度患者冠状动脉疾病风险评估预测模型的制定

Q4 Medicine
Karan Ramu, A. V., Ananya Rajagopal, R. Varyani, P. Kini, Prakash Kumar, S. Sabat
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引用次数: 1

摘要

背景和目的:变形杆菌是几种胃肠道疾病的主要致病菌,已知在胃肠道不同部位提供的广泛pH范围内存活。细菌酶胆碱TMA裂解酶在一些机会性肠道共栖物中发现,催化胆碱转化为三甲胺,三甲胺是促动脉粥样硬化代谢物三甲胺N氧化物的前体。本研究评估了冠状动脉疾病患者肠道变形杆菌的致病潜力。我们还试图建立一个简单的预测模型来评估冠状动脉疾病的危险因素,使用印度患者的样本。材料与方法:纳入冠心病患者14例,对照组6例。设计最佳条件,并遵循标准化方案,体外培养变形杆菌,分离目标蛋白胆碱TMA裂解酶。采用红外光谱法和紫外分光光度法分别测定胆碱和三甲胺N氧化物水平。最后,通过受试者工作特征分析和多元logistic回归建立了整个模型和三甲胺N氧化物的预测能力。结果:该蛋白在pH值7.4 ~ 9范围内具有最佳活性,细菌生长良好。定量分析显示,冠状动脉疾病患者三甲胺N氧化物水平(1.81µM)显著高于对照组(0.86µM)。结论:碱性条件下的最佳活性表明变形杆菌在冠状动脉疾病的发展过程中具有很强的病理潜能。该预测模型可作为医学界评估冠状动脉疾病危险因素的有用工具。et al。变形杆菌通过胆碱代谢途径对三甲胺N-氧化物产生的影响及预测方法的制定
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of Proteus sp. on Trimethylamine N- Oxide production via the Choline Metabolism Pathway and the Formulation of a Predictive Model to Assess the Risk of Coronary Artery Disease in Indian Patients
Background and Aim: Proteus bacteria, a key contributor to several gastrointestinal diseases, is known to survive in the wide pH range offered by different locations of the GI tract. The bacterial enzyme Choline TMA Lyase found in several opportunistic gut commensals catalyzes choline conversion to trimethylamine, a precursor of the pro-atherosclerotic metabolite trimethylamine N oxide. This study evaluates the pathogenic potential of Proteus gut bacteria in patients with coronary artery disease. We also sought to create a simple predictive model for assessing risk factors of coronary artery disease using a sample of Indian patients. Materials and Methods: The study included 14 patients with coronary artery disease and 6 controls. Optimal conditions were devised, and standardized protocols were followed to culture Proteus bacteria in vitro and isolate the protein of interest, Choline TMA Lyase. FTIR analysis and UV spectrophotometry were employed to quantify choline and trimethylamine N oxide levels, respectively. Finally, receiver operating characteristic analysis and multivariate logistic regression established the predictive power of the entire model and trimethylamine N oxide. Results: The findings demonstrated an optimum activity of this protein and the bacterial growth in the pH range of 7.4 - 9. Quantitative analysis showed trimethylamine N oxide levels to be significantly higher in coronary artery disease patients (1.81 µM) than in controls (0.86 µM). Conclusion: Optimum activity in the alkaline condition indicates the strong pathological potential of Proteus bacteria in the progression of coronary artery disease. The prediction model can serve as a helpful tool within the medical community to assess the risk factors for coronary artery disease. et al. Influence of Proteus spp. on Trimethylamine N- Oxide production via the Choline Metabolism Pathway and the Formulation of a Predictive
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来源期刊
Iranian Journal of Medical Microbiology
Iranian Journal of Medical Microbiology Medicine-Infectious Diseases
CiteScore
1.60
自引率
0.00%
发文量
70
审稿时长
8 weeks
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