{"title":"氟对黄原菌氧化应激的诱导作用。","authors":"Ouahiba Benhamada, Nabila Benhamada, E. Leghouchi","doi":"10.21448/ijsm.1136546","DOIUrl":null,"url":null,"abstract":"In our work we were interested in the toxicity of fluorine on the various parameters of stress: chlorophyll, proteins, and antioxidant system in the lichen Xanthoria parietina (L.) Th. Fr., and for this purpose, lichen thalli were treated by sodium fluoride (NaF) at concentrations of 0, 0.5, 1.0, 5.0 and 10.0 mM, for time scale 0, 24, 48 and 96 h. The analysis results obtained revealed that all the parameters evaluated showed significant variations compared to those of the controls. From the analysis results obtained, it was noted that chlorophyll a (Ca), chlorophyll b (Cb) and total chlorophyll (Ca+b) decreased correlating with exposure times to NaF (r = -0.785, p < 0.001; r = -0.955, p < 0.001; r = -0.899, p < 0.001, respectively), with a significant increase of Ca/b ratio (p = 0.00572**) showing that Cb was more affected than Ca. However, hydrogen peroxide (H2O2) increased (r = 0.949, p < 0.001). In correlation with NaF concentrations, Glutathione (GSH) increased (r = 0.969, p < 0.001), while proteins decreased (r = -0.872, p < 0.001). Furthermore, results showed that catalase activity (CAT) increased correlating with increasing exposure time of X. parietina to increasing concentrations of NaF. Long-term exposure (48 h -96 h) caused a significant decrease in GSH content (p = 0.02*) followed by total destruction at time 96 h.","PeriodicalId":14437,"journal":{"name":"International Journal of Secondary Metabolite","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Oxidative stress induced by fluorine in Xanthoria parietina (L.) Th. Fr.\",\"authors\":\"Ouahiba Benhamada, Nabila Benhamada, E. Leghouchi\",\"doi\":\"10.21448/ijsm.1136546\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In our work we were interested in the toxicity of fluorine on the various parameters of stress: chlorophyll, proteins, and antioxidant system in the lichen Xanthoria parietina (L.) Th. Fr., and for this purpose, lichen thalli were treated by sodium fluoride (NaF) at concentrations of 0, 0.5, 1.0, 5.0 and 10.0 mM, for time scale 0, 24, 48 and 96 h. The analysis results obtained revealed that all the parameters evaluated showed significant variations compared to those of the controls. From the analysis results obtained, it was noted that chlorophyll a (Ca), chlorophyll b (Cb) and total chlorophyll (Ca+b) decreased correlating with exposure times to NaF (r = -0.785, p < 0.001; r = -0.955, p < 0.001; r = -0.899, p < 0.001, respectively), with a significant increase of Ca/b ratio (p = 0.00572**) showing that Cb was more affected than Ca. However, hydrogen peroxide (H2O2) increased (r = 0.949, p < 0.001). In correlation with NaF concentrations, Glutathione (GSH) increased (r = 0.969, p < 0.001), while proteins decreased (r = -0.872, p < 0.001). Furthermore, results showed that catalase activity (CAT) increased correlating with increasing exposure time of X. parietina to increasing concentrations of NaF. Long-term exposure (48 h -96 h) caused a significant decrease in GSH content (p = 0.02*) followed by total destruction at time 96 h.\",\"PeriodicalId\":14437,\"journal\":{\"name\":\"International Journal of Secondary Metabolite\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-03-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Secondary Metabolite\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21448/ijsm.1136546\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Secondary Metabolite","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21448/ijsm.1136546","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
在我们的工作中,我们对氟对地衣叶绿素、蛋白质和抗氧化系统的各种胁迫参数的毒性感兴趣。Th。在0、0.5、1.0、5.0和10.0 mM浓度的氟化钠(NaF)处理地衣,时间尺度分别为0、24、48和96 h。分析结果表明,与对照组相比,所有参数均有显著差异。分析结果表明,叶绿素a (Ca)、叶绿素b (Cb)和总叶绿素(Ca+b)随NaF暴露时间的增加而降低(r = -0.785, p < 0.001);R = -0.955, p < 0.001;r = -0.899, p < 0.001), Ca/b比值显著升高(p = 0.00572**),表明Cb比Ca受影响更大,而过氧化氢(H2O2)升高(r = 0.949, p < 0.001)。与NaF浓度相关,谷胱甘肽(GSH)含量升高(r = 0.969, p < 0.001),蛋白质含量降低(r = -0.872, p < 0.001)。此外,过氧化氢酶活性(CAT)随NaF浓度的增加而增加。长期暴露(48 h -96 h)导致谷胱甘肽含量显著降低(p = 0.02*),随后在96 h发生完全破坏。
Oxidative stress induced by fluorine in Xanthoria parietina (L.) Th. Fr.
In our work we were interested in the toxicity of fluorine on the various parameters of stress: chlorophyll, proteins, and antioxidant system in the lichen Xanthoria parietina (L.) Th. Fr., and for this purpose, lichen thalli were treated by sodium fluoride (NaF) at concentrations of 0, 0.5, 1.0, 5.0 and 10.0 mM, for time scale 0, 24, 48 and 96 h. The analysis results obtained revealed that all the parameters evaluated showed significant variations compared to those of the controls. From the analysis results obtained, it was noted that chlorophyll a (Ca), chlorophyll b (Cb) and total chlorophyll (Ca+b) decreased correlating with exposure times to NaF (r = -0.785, p < 0.001; r = -0.955, p < 0.001; r = -0.899, p < 0.001, respectively), with a significant increase of Ca/b ratio (p = 0.00572**) showing that Cb was more affected than Ca. However, hydrogen peroxide (H2O2) increased (r = 0.949, p < 0.001). In correlation with NaF concentrations, Glutathione (GSH) increased (r = 0.969, p < 0.001), while proteins decreased (r = -0.872, p < 0.001). Furthermore, results showed that catalase activity (CAT) increased correlating with increasing exposure time of X. parietina to increasing concentrations of NaF. Long-term exposure (48 h -96 h) caused a significant decrease in GSH content (p = 0.02*) followed by total destruction at time 96 h.