响应面法优化金萱夏绿茶中表没食子儿茶素没食子酸酯的提取工艺

IF 1.2 4区生物学 Q2 Agricultural and Biological Sciences

Journal of Applied Botany and Food Quality-Angewandte Botanik Pub Date : 2018-07-10 DOI:10.5073/JABFQ.2018.091.022

Xiang Limin, Shunshun Pan, Xingfei Lai, Lingli Sun, Zhigang Li, Qiuhua Li, Ya-hui Huang, Sun Shili

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引用次数: 2

摘要

研究了金选夏季绿茶中表没食子儿茶素-3-没食子酸酯（EGCG）的提取条件及其对人癌症SGC-7901细胞的抗肿瘤活性。在单因素实验的基础上，采用Box-Behnken设计和响应面法对热水提取条件进行了优化。确定了EGCG的最佳提取条件为：提取温度85°C，提取时间34min，水茶比41mL/g，溶液pH 6，提取两次。在这些条件下，EGCG的实验提取产率值为33.82mg/g，与预测值相比没有显著差异。结果表明，回归模型适用于金萱夏绿茶EGCG的提取。在最佳条件下制备的夏季绿茶提取物对人癌症SGC-7901细胞的抗肿瘤活性高于传统泡茶方法制备的绿茶提取物。

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Optimization of brewing conditions in epigallocatechin-3-gallate (EGCG) extraction from Jinxuan summer green tea by response surface methodology

The extraction conditions of epigallocatechin-3-gallate (EGCG) from Jinxuan summer green tea and antitumor activity against human gastric cancer SGC-7901 cells of the green tea extracts were investigated. On the basis of a single factor experiment, Box-Behnken design and response surface methodology were employed to optimize the hot water extraction conditions. The optimal extraction conditions for EGCG were determined as: extraction temperature of 85 °C, extraction time of 34 min, water-tea ratio of 41 mL/g, a solution of pH 6, and extraction twice. Under these conditions, the experimental extraction yield value of EGCG was 33.82 mg/g, which was not significantly different in comparison to predicted values. The results indicated that the regression models were suitable for the EGCG extraction from Jinxuan summer green tea. The summer green tea extract prepared under the optimal conditions had a higher antitumor activity against human gastric cancer SGC-7901 cells than the green tea extract made with traditional tea brewing method.

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来源期刊

Journal of Applied Botany and Food Quality-Angewandte Botanik 生物-植物科学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： The Journal of Applied Botany and Food Quality is the Open Access journal of the German Society for Quality Research on Plant Foods and the Section Applied Botany of the German Botanical Society. It provides a platform for scientists to disseminate recent results of applied plant research in plant physiology and plant ecology, plant biotechnology, plant breeding and cultivation, phytomedicine, plant nutrition, plant stress and resistance, plant microbiology, plant analysis (including -omics techniques), and plant food chemistry. The articles have a clear focus on botanical and plant quality aspects and contain new and innovative information based on state-of-the-art methodologies.