INSULIN HORMONE LEVELS IN DIABETIC RAT SERUM (Rattus norvegicus) GIVEN THE ETHYL ACETATE FRACTION OF BITTER MELON FRUIT (Momordica charantia L.) WITH ELISA METHOD

Prevalence of diabetes mellitus according to  WHO  been projected increase from 2.8% in 2000 to 4.4% in 2030. Various studies have been conducted to find alternative sources of antidiabetic therapy, Momordica charantia known has mimetic insulin compounds. Ethanol extract of Momordica charantia reported show optimal results in reducing blood glucose levels and stimulating pancreatic beta cells in secreting insulin hormone. This study aims to determine effect of bitter melon extract on insulin levels in streptozotosin-induced rat.  This study consisted in 3 groups test namely streptozotosin-induced rat, streptozotosin-induced rat given ethyl acetate fraction of Momordica charantia extract, and not induced-streptozotosin rats. Insulin levels measured by  ELISA method. Data analysis used Unpaired T-test considered if (p value >0,05). Research result show that there is not different between the insulin levels of streptozotosin-induced rat with streptozotosin-induced rat given ethyl acetate fraction of Momordica charantia extract group (p=0,081). There is not different between  insulin levels of streptozotosin-induced rat given ethyl acetate fraction of Momordica charantia extract with not induced-streptozotosin rats (p=0,505). Conclusion. There is not different between insulin levels of streptozotosin-induced rat, streptozotosin-induced rat given ethyl acetate fraction of Momordica charantia extract and not induced streptozotosin rats group.