RAPD-PCR技术检测桃蝇、带状双峰虫和雌性γ射线辐射后的遗传变异

IF 0.4 4区农林科学 Q4 ENTOMOLOGY

Entomological News Pub Date : 2022-11-01 DOI:10.3157/021.130.0304

R. Sayed, N. Zahran, A. Hamza

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引用次数: 0

摘要

摘要。带状细菌（Saunders）是一种破坏性的多食性昆虫，寄生在世界各地的各种水果和蔬菜中。目前，辐射技术作为一种安全的控制工具被广泛使用。因此，本研究旨在探索伽马辐射对带状B.zonata成年雌性的影响。用雌性消毒和亚消毒剂量的γ射线照射成年蛹。与未经辐照的雌性相比，暴露于伽马射线引起成年雌性DNA模式的改变，辐照雌性RAPD-PCR扩增模式中出现一些额外的条带和其他条带的缺乏。

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Genetic Variation in Peach Fruit Fly, Bactrocera zonata, Females Irradiated with Gamma Radiation as Revealed by RAPD-PCR Technique

ABSTRACT. Bactrocera zonata (Saunders) is a destructive polyphagous insect that infests various fruits and vegetables worldwide. Nowadays, radiation technology is widely used as a safe tool for controlling it. Therefore, this study was designed to explore the effect of gamma radiation on adult females of B. zonata. Full-grown pupae were irradiated with female sterilizing and substerilizing doses of gamma irradiation. Exposure to gamma radiation-induced alterations in DNA patterns of adult females, the appearance of some extra bands and the deficiency of others in the RAPD-PCR amplification patterns of irradiated females when compared with unirradiated females.

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来源期刊

Entomological News 生物-昆虫学

CiteScore

1.10

自引率

25.00%

发文量

期刊介绍： Contributions are accepted on any of the aspects of systematics and taxonomy of insects. This includes: taxonomic revisions, phylogenetics, biogeography and faunistics, and descriptive morphology of insects. Taxonomic papers describing single species are acceptable if such a study is sufficiently justified, however, comprehensive studies that provide a comparative, integrated study of insect systematics and taxonomy will get priority.