人Homeobox TGIFLX调节结直肠癌癌症细胞系中CDX1、CDX2和OCT1基因的表达

IF 0.4 Q4 ONCOLOGY

Middle East Journal of Cancer Pub Date : 2021-06-26 DOI:10.30476/MEJC.2021.86467.1345

Soroosh Shahryarhesami, M. Heidari, Masoud Heidari, N. Sadighi

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引用次数: 0

摘要

背景:同源域转录调节蛋白是由同源盒(HOX)基因编码的，在正常发育和致癌过程中都起着重要作用。先前的研究表明，HOX基因的表达在许多肿瘤中是不受调控的，并且这种表达是基于产生的胚胎起源组织和肿瘤部位对每种癌症特异性的。方法:在体外研究中，比较10种不同分化阶段的人结直肠癌细胞系中HOXA10、CDX1、CDX2、TGIFLX、TGIFLY和OCT1基因的表达水平。随后，在SW948细胞系中分析TGIFLX sirna介导的敲低对CDX1、CDX2和OCT1基因表达水平的影响。结果:所得结果显示，这些同源盒基因在不同的结直肠癌细胞系中表达存在差异。此外，sirna介导的tgflx敲低导致CDX1、CDX2和OCT1表达水平升高。结论:TGIFLX在CDX1、CDX2和OCT1基因的上游调控中发挥重要作用。

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Human Homeobox TGIFLX Regulates CDX1, CDX2, and OCT1 genes Expression in Colorectal Cancer Cell Lines

Background: Homeodomain transcriptional regulatory proteins, which are encoded by Homeobox (HOX) genes, play critical roles in both normal development and carcinogenesis. Previous studies have shown that the expression of HOX genes is deregulated in numerous tumors and this expression is specific to each cancer based on the arising embryonic origin tissue and the site of tumor. Method: In this in vitro study, the expression levels of HOXA10, CDX1, CDX2, TGIFLX, TGIFLY, and OCT1 genes were compared across 10 different human colorectal cancer cell lines with different differentiation stages. Subsequently, the effect of TGIFLX siRNA-mediated knockdown on the expression levels of CDX1, CDX2, and OCT1 genes was analyzed in SW948 cell line. Results: The obtained results revealed that these homeobox genes were differentially expressed in different colorectal cancer cell lines. Furthermore, the siRNA-mediated knockdown of TGIFLX led to higher levels of CDX1, CDX2, and OCT1 expression. Conclusion: Our data suggested that TGIFLX plays an important role in the upstream regulation of CDX1, CDX2, and OCT1 genes.

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来源期刊

Middle East Journal of Cancer ONCOLOGY-

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Middle East Journal of Cancer (MEJC) is an international peer-reviewed journal which aims to publish high-quality basic science and clinical research in the field of cancer. This journal will also reflect the current status of research as well as diagnostic and treatment practices in the field of cancer in the Middle East, where cancer is becoming a growing health problem. Lastly, MEJC would like to become a model for regional journals with an international outlook. Accordingly, manuscripts from authors anywhere in the world will be considered for publication. MEJC will be published on a quarterly basis.