全内反射荧光显微镜中基于反射镜的二维各向同性照明

IF 1.1 Q4 OPTICS
Sarina Yaghoubi, B. Sajad, S. Tavaddod
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引用次数: 0

摘要

在全内反射荧光显微镜中,激发光的强度存在三维各向异性。在基于棱镜的全内反射荧光显微镜中使用反射镜,我们提出了一种非常低成本的方法来降低激发光的各向异性。从理论上讲,我们获得了样品内部透射光的电场,它是反射镜旋转角、入射光束偏振度以及棱镜、浸油、盖玻片和样品折射率的函数。通过使用市售的折射率,建议镜的角度和入射光的偏振度的适当的实际组合,以在全内反射荧光显微镜的样品平面处提供仅仅2D各向同性激发光。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A mirror-based 2D isotropic illumination in total internal reflection fluorescence microscopy
In a total internal reflection fluorescence microscope, there is a 3D anisotropy in intensity of the excitation light. Using a mirror in a prism-based total internal reflection fluorescence microscope, we propose a very low-cost method to decrease the anisotropic property of the excitation light. Theoretically, we obtained the electric field of the transmitted light inside the sample as a function of the rotational angle of the mirror, polarization degree of the incident beam, and the refractive indices of the prism, immersion oil, cover glass, and sample. By using the refractive indices that are commercially available, proper practical combinations of the angle of mirror and polarization degree of the incident light are suggested to provide a merely 2D isotropic excitation light at the sample plane of a total internal reflection fluorescence microscope.
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CiteScore
3.50
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