使用可变形和电子透射膜对溶液中未固定组织进行实时扫描电子显微镜检查

IF 1.5 4区工程技术 Q3 MICROSCOPY

Microscopy Pub Date : 2022-06-17 DOI:10.1093/jmicro/dfac030

Seine A. Shintani, S. Yamaguchi, H. Takadama

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引用次数: 0

摘要

摘要用扫描电子显微镜很难观察浸泡在溶液中的生物组织的结构和运动。为了进行这样的观察，我们制作了一种高度可变形和电子透射的聚酰亚胺薄膜，它可以承受高真空电子柱和大气压样品室之间的压力差。利用这种薄膜，我们使用扫描电子显微镜测量了浸泡在生理溶液中的离体小鼠心脏收缩纤维的内在精细结构和运动。我们的测量结果表明，切除的心脏是一个动态组织，基于三维力平衡进行松弛振荡。

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Real-time scanning electron microscopy of unfixed tissue in the solution using a deformable and electron-transmissive film

Abstract It is difficult to use scanning electron microscopy to observe the structure and movement of biological tissue immersed in the solution. To enable such observations, we created a highly deformable and electron-transmissive polyimide film that can withstand the pressure difference between the high-vacuum electron column and the atmospheric-pressure sample chamber. With this film, we used scanning electron microscopy to measure the intrinsic fine structure and movement of the contractile fibers of excised mouse heart immersed in physiological solutions. Our measurements revealed that the excised heart is a dynamic tissue that undergoes relaxation oscillation based on a three-dimensional force balance.

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来源期刊

Microscopy Physics and Astronomy-Instrumentation

CiteScore

3.30

自引率

11.10%

发文量

期刊介绍： Microscopy, previously Journal of Electron Microscopy, promotes research combined with any type of microscopy techniques, applied in life and material sciences. Microscopy is the official journal of the Japanese Society of Microscopy.