精油对甜瓜枯萎病的抗真菌作用

IF 0.9 4区农林科学 Q3 AGRONOMY

Revista Caatinga Pub Date : 2023-09-01 DOI:10.1590/1983-21252023v36n301rc

A. E. D. Sousa, Rafaela P. Melo, P. R. Gagliardi, G. Nunes, A. H. Oster, E. Silva

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引用次数: 0

摘要

摘要本工作的目的是确定精油（Lippia sidoides Cham.，Ocimum gratisimum L.，Cymbopogon citratus Stapf，Ocimum-selloi Bent.，Citrus sinensis L.，Ocimum.micranthum Willd.，Ocimumsp.，and Piper aduncum L.）的成分，并在体外和体内评价其对由真菌苍白镰刀菌引起的瓜果镰刀菌腐病的控制作用。采用加氢蒸馏法得到精油，并用GCMS和GC-FID测定其化学成分。在体外实验中评估了每种精油（浓度为0、500、1500和3000µL L-1）对真菌菌丝生长的影响。通过probit法测定了抑制50%菌丝生长的有效浓度（EC50）；还计算了菌丝生长指数（MGI）。在瓜果（Galia）的体外和体内实验中，评价了具有较高抑制苍白球孢菌丝生长潜力的精油对真菌孢子萌发的抑制作用。精油的化学成分分析能够识别出不同数量的化合物，其中以单萜类化合物为主。西氏乳杆菌（L.sidoides）、灰葡萄球菌（O.grassimum）、柠檬曲霉（C.citratus）和薇甘菊（O.micranthum）的精油对苍白葡萄菌丝生长和孢子萌发具有较高的抑制作用，因此，它们是开发商业杀菌剂的有前景的原料，主要用于控制苍白葡萄采后腐烂。

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Antifungal action of essential oils against Fusarium rot in melon

ABSTRACT The objective of this work was to determine the composition and evaluate in vitro and in vivo effects of essential oils (Lippia sidoides Cham., Ocimum gratissimum L., Cymbopogon citratus Stapf., Ocimum selloi Benth., Citrus sinensis L., Ocimum micranthum Willd., Ocimum sp., and Piper aduncum L.) on the control of Fusarium rot in melon fruits, caused by the fungus Fusarium pallidoroseum. The essential oils were obtained by hydrodistillation and their chemical composition was determined by GCMS and GC-FID. The effect of each essential oil (concentrations of 0, 500, 1500, and 3000µL L-1) on the fungal mycelial growth was evaluated in in vitro experiment. The effective concentration that inhibited 50% of mycelial growth (EC50) was determined through the probit method; mycelial growth index (MGI) was also calculated. The essential oils with higher potential for inhibiting mycelial growth of F. pallidoroseum were evaluated for their inhibitory effect on the fungus spore germination in in vitro and in vivo experiments using melon fruits (variety Galia). Chemical composition analysis of essential oils enabled the identification of varying amounts of chemical compounds, with predominance of monoterpenes. The essential oils of L. sidoides, O. gratissimum, C. citratus, and O. micranthum presented higher inhibiting effects on F. pallidoroseum mycelial growth and spore germination, therefore, they are promising raw materials for the development of commercial fungicides, mainly for controlling postharvest rot caused by F. pallidoroseum.

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来源期刊

Revista Caatinga AGRONOMY-

CiteScore

2.10

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： A Revista Caatinga é uma publicação científica que apresenta periodicidade trimestral, publicada pela Pró-Reitoria de Pesquisa e Pós-Graduação da Universidade Federal Rural do Semi-Árido – UFERSA, desde 1976. Objetiva proporcionar à comunidade científica, publicações de alto nível nas áreas de Ciências Agrárias e Recursos Naturais, disponibilizando, integral e gratuitamente, resultados relevantes das pesquisas publicadas.