发布信息TOC

Q2 Pharmacology, Toxicology and Pharmaceutics
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引用次数: 0

摘要

封面:在kaspricet al. (https://doi.org/10.1002/cpph.55)中,图像显示了对皮肤切片的分析。(A) mCOL7vWFA2免疫兔滴度测定。正常小鼠皮肤冷冻切片用1:4 000、1:8 000、1:16 000和1:32 000稀释的血清孵育,然后按照基本方案3用FITC-AffiniPure驴抗兔IgG染色。由于血清特异性地与皮肤的真皮-表皮连接处结合,可见一条典型的绿线(箭头)。在最低测试浓度下可见的线决定滴度(这里为1:8 000)。(B) H&E染色分析。抗体转移诱导EBA第一次注射IgG后12天典型耳切片。左上为轻度炎症,增厚较少,仅有少量炎症细胞。其他图片显示典型的表皮增厚和大量炎症。箭头表示真皮与表皮交界处形成分裂;**表示耳软骨。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Issue Information TOC

Issue Information TOC

Cover: In Kasprick et al. (https://doi.org/10.1002/cpph.55), the image shows analysis of skin sections. (A) Determination of titer from rabbits immunized with mCOL7vWFA2. Cryosections of normal mouse skin were incubated with serum diluted 1:4,000 1:8,000, 1:16,000, and 1:32,000 and then stained with FITC-AffiniPure donkey anti-rabbit IgG as described in Basic Protocol 3. As the serum specifically binds to the dermal-epidermal junction of the skin, a typical green line is visible (arrows). The line visible at the lowest tested concentration determines the titer (here, 1:8,000). (B) Analysis of H&E staining. Typical ear sections 12 days after first IgG injection in antibody transfer–induced EBA. Upper left picture shows a mild inflammation with less thickening and only a few inflammatory cells. Other pictures show typical thickening of the epidermal layers and massive inflammation. Arrows indicate split formation at dermal-epidermal junction; **indicates cartilage of the ear.

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Current Protocols in Pharmacology
Current Protocols in Pharmacology Pharmacology, Toxicology and Pharmaceutics-Pharmacology
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