基于uplc - q - tof - ms的无菌条件下菌核菌分泌次生代谢物的非靶向研究

Gupta Navin Chandra, Arora Shaweta, Kundu Aditi, S. Pankaj, Rao Mahesh, Bhattacharya Ramcharan
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引用次数: 1

摘要

茎腐病已在全球范围内成为对油菜产量和种子质量的主要威胁。广义致病病原体核盘菌(Sclerotinia sclerodorum,Lib.)de Bary在其攻击性和致病性方面表现出很大的变异性。揭示病原体在宿主-病原体相互作用中的代谢特征和信号传导成分是了解宿主对疾病耐药性的基础。在本研究中,使用非靶向高分辨率UPLC-QTOF-ESI-MS/MS鉴定了核盘菌致病菌株在无菌培养条件下释放的代谢产物。对核盘菌培养物的乙酸乙酯提取物进行分析,发现了十种主要的次生代谢产物,即sclerin、scleronin-B、sclerone、黑色素、bostrycoidin、botcinin-D、botcinin-A、胶质病毒蛋白、巩膜酰胺和botcinic acid。随后的六种代谢产物首次在核盘菌病原体的培养提取物中被报道。根据色谱图中重叠和独特的信息峰,在系统发育分析中将6个核盘菌菌株分为三个主要分支。基于代谢谱的聚类并不能证实基于宿主形态学或毒力差异的多样性。研究结果表明,在无菌条件下分泌的代谢产物因其生长和发育阶段而异,不一定是其对宿主不同攻击性和毒力的决定因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
UPLC-Q-TOF-MS-based untargeted studies of the secondary metabolites secreted by Sclerotinia sclerotiorum under the axenic condition
The stem rot disease has emerged globally as a major threat to oilseed Brassica's productivity and seed quality. The generalist causal pathogen Sclerotinia sclerotiorum (Lib.) de Bary shows large variability in their aggressiveness and pathogenicity. Revealing the pathogen's metabolic profile and signaling components in host-pathogen interaction is fundamental in understanding host resistance to the disease. In this study, the metabolites released by the pathogenic strains of S. sclerotiorum under the axenic culture have been identified using the untargeted high-resolution UPLC-QTOF-ESI-MS/MS. The analysis of the ethyl acetate extracts of the S. sclerotiorum culture revealed ten major secondary metabolites namely, sclerin, sclerotinin-B, sclerone, melanin, bostrycoidin, botcinin-D, botcinin-A, gliovirin, scleramide, and botcinic acid. The later six metabolites are being reported for the first time in the culture extract of the S. sclerotiorum pathogen. Based on the overlapping and unique informative peaks in the chromatograms, the six S. sclerotiorum strains were grouped into three major clades in the phylogenetic analysis. The clustering based on metabolic profiles does not substantiate the diversity based on morphology or virulence differences over the host. The findings of the study signified the metabolites secreted under the axenic conditions are varies based on their growth and developmental stages and may not necessarily be the determining factors for their differential aggressiveness and virulence to their host.
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