豚鼠胎成骨细胞接种制备腺松质骨复合异种移植物

IF 0.1 0 VETERINARY SCIENCES
Mohar Singh, A. Pawde, K. E, K. Sharun, Surendra D. S., Atal Kumar, Reena Mukherjee, K. P. Singh, Amarpal .
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引用次数: 0

摘要

异种骨移植被认为是修复临界骨缺损的一种替代策略。本研究规范了用豚鼠胚胎成骨细胞播种和冷冻保存脱细胞丁醛松质骨的技术,以制备复合骨异种移植。将扩增的成骨细胞植于脱细胞丁醛松质骨基质上制备复合骨移植物。采用扫描电镜(SEM)和组织学检查对胚胎成骨细胞种子支架、脱细胞骨支架和天然牛骨进行分析。复合骨异种移植物在- 80°C的10%甘油中冷冻保存3个月,并评估解冻后的生存能力。体外实验结果表明,脱细胞支架的播种量足够,成骨细胞在脱细胞骨基质孔隙内具有良好的粘附和增殖能力。组织学上,与新鲜种子和冷冻保存的支架相比,脱细胞组胶原基质的完整性得到了最好的保存。成骨细胞植入的脱细胞丁醛松质骨异种移植物在- 80°C的无菌试管中,含有10%甘油作为冷冻保护剂,可保存3个月,解冻后评估细胞活力充足。
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Development of composite bubaline cancellous bone xenografts by seeding guinea pig fetal osteoblasts
: Xenografts are considered as an alternative strategy to restore a critical sized bone defect. The present study was conducted to standardize the technique for seeding and cryopreservation of decellularized bubaline cancellous bone with Guinea pig fetal osteoblast for the development of a composite bone xenograft. The composite bone grafts were prepared by seeding the expanded osteoblasts on the acellular bubaline cancellous bone matrix. The foetal osteoblast seeded scaffold, acellular bone scaffold, and native bovine bone were analyzed using scanning electron microscopy (SEM) and histological examination. Composite bone xenograft was cryopreserved in 10% glycerol at - 80°C for three months and evaluated for post-thaw viability. Findings from the in vitro study suggested that the seeding of acellular scaffold was adequate, and osteoblasts had good adhesion and proliferation inside pores of acellular bone matrix. Histologically the integrity of collagen matrix was best preserved in the acellular group as compared to the freshly seeded and cryopreserved scaffold. The osteoblast seeded decellularized bubaline cancellous bone xenografts can be preserved for three months with adequate cell viability in the post-thaw evaluation at – 80°C in sterile tubes containing 10% glycerol as the cryoprotectant.
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来源期刊
CiteScore
1.30
自引率
0.00%
发文量
16
审稿时长
20 weeks
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