Green chemistry approach to the synthesis of zinc nanoparticles using Cyperus rotundus rhizome extract for the treatment of lung well-differentiated bronchogenic adenocarcinoma

Abstract In the present study, zinc oxide nanoparticles were green-synthesized using the aqueous extract of the rhizomes of Cyperus rotundus. The chemical methods EDX, FE-SEM, XRD, UV-Vis., and FT-IR analysis were used to characterize ZnONPs@C. rotundus were characterized by analytical techniques including. The FE-SEM image revealed a spherical shape for the nanoparticles in a size range of 38.05 to 75.41 nm and 33.09 nm was calculated for ZnONPs@ C. rotundus crystal size using the XRD results. MTT assay was used on common lung well-differentiated bronchogenic adenocarcinoma cell line i.e. HLC-1 to survey the cytotoxicity and anti-lung well-differentiated bronchogenic adenocarcinoma effects of the synthesized nanoparticles. To determine the antioxidant properties of the synthesized nanoparticles, the DPPH test was used in the presence of butylated hydroxytoluene as the positive control. The synthesized nanoparticles had very low cell viability and high anti-lung well-differentiated bronchogenic adenocarcinoma activities dose-dependently against the HLC-1 cell line without any cytotoxicity on the normal cell line (HUVEC). The synthesized nanoparticles inhibited half of the DPPH molecules at the concentration of 41 µg/mL. Maybe significant anti-human lung well-differentiated bronchogenic adenocarcinoma potentials of the synthesized nanoparticles against common human lung well-differentiated bronchogenic adenocarcinoma cell lines are linked to their antioxidant activities.