巴基斯坦拉合尔不同医院对耐甲氧西林金黄色葡萄球菌诊断的差异

IF 0.3 Q3 MEDICINE, GENERAL & INTERNAL

International Medical Journal Malaysia Pub Date : 2020-11-20 DOI:10.31436/IMJM.V8I2.750

T. Romeeza, A. Nadeem, J. Nauman, A. Afia, R. Kunwal

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引用次数: 1

摘要

摘要简介:金黄色葡萄球菌是一种引起广泛感染的病原体。在抗生素的选择压力下，它改变了自己的基因组。甲氧西林耐药常用Kirby-Bauer盘片扩散法测定，敏感性和特异性较低。因此，采用抗PBP-2a胶乳凝集试验来确认这些标记为MRSA的人的身份。材料与方法:在拉合尔不同医院采集100份经Kirby-Bauer扩散法鉴定为MRSA的细菌样本。结果:经生化鉴定，64株(64%)为金黄色葡萄球菌，其余36株(36%)为凝固酶阴性葡萄球菌。进一步的血清学鉴定显示，64株金黄色葡萄球菌中，50株(78%)为MRSA, 14株(22%)为MSSA。结论:因此，总体而言，50%的菌株被误诊为MRSA。对抗生素耐药性的误诊可能导致对这些患者不适当的药物处方。关键词:MRSA, MSSA，表皮葡萄球菌，凝集

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Discrepancies in the diagnosis of methicillin resistant Staphylococcus aureus among different hospitals in Lahore, Pakistan

ABSTRACT Introduction: Staphylococcus aureus is a pathogen causing a wide range of infections. It changes its genome under the selection pressure of antibiotics. Methicillin resistance is commonly determined by Kirby-Bauer disc diffusion method which has low sensitivity and specificity. Thus, anti PBP-2a latex agglutination test was used to confirm the identity of those labeled as MRSA. Materials and Methods: One hundred bacterial samples characterized as MRSA according to Kirby-Bauer diffusion method were collected from different hospitals in Lahore. Results: Biochemical characterization of these MRSA isolates revealed that only 64 (64%) were actually S. aureus while the other 36 isolates (36%) were coagulase negative staphylococci. Further serological characterization revealed that among the 64 isolates of S. aureus, 50 (78%) were MRSA while 14 (22%) were MSSA. Conclusion: Therefore, overall, 50% of the bacterial strains were misidentified as MRSA. Misdiagnosis of antibiotic resistance may lead to an inappropriate prescription of medicines to these patients. KEY WORDS: MRSA, MSSA, Staphylococcus epidermidis, Agglutination

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来源期刊

International Medical Journal Malaysia Medicine-Medicine (all)

CiteScore

0.40

自引率

0.00%

发文量

期刊介绍： International Medical Journal Malaysia (IMJM) is the official journal of the Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia. It serves primarily as a forum for education and intellectual discourse for health professionals namely in clinical medicine but covers diverse issues relating to medical ethics, professionalism as well as medical developments and research in basic medical sciences. It also serves the unique purpose of highlighting issues and research pertaining to the Muslim world. Contributions to the IMJM reflect its international and multidisciplinary readership and include current thinking across a range of specialties, ethnicities and societies.