{"title":"预防和同时接种解淀粉芽孢杆菌(Fukumoto)菌株对灰葡萄孢分生孢子萌发的影响。","authors":"A. Hamdache, M. Ezziyyani, A. Lamarti","doi":"10.6018/ANALESBIO.40.08","DOIUrl":null,"url":null,"abstract":"\nBotrytis cinerea es un patógeno fúngico necrotrófico de varias plantas distribuido en todo el mundo. En el presente estudio se analizó el efecto de nueve aislados bacterianos sobre la germinación de conidios de B. cinerea. Los aislados se identificaron como Bacillus amyloliquefaciens. Para evaluar la eficacia de los aislados, se realizaron confrontaciones duales tanto preventivas como simultáneas con los conidios de B. cinerea. Los resultados mostraron que cinco aislados fueron más eficaces cuando se co-inocularon simultáneamente con el patógeno. Cuatro aislados fueron más eficaces cuando se inocularon previamente cuatro horas antes el patógeno. La cepa denominada B24, mostró una inhibición de (84.04%) en concentraciones muy bajas (3x103 UFC/ml) y la cepa RA9 fue la cepa menos eficaz.\n\n\nBotrytis cinerea is a necrotrophic fungal plant pathogen distributed worldwide. In the current study, the effect of nine bacterial isolates, on germination of B. cinerea conidia were studied. The nine isolates were identified as Bacillus amyloliquefaciens. The efficacy of isolates was tested, at different concentrations, in preventive and simultaneous inoculations with B. cinerea conidia. Results showed that five Bacillus isolates were more effective when co-inoculated simultaneously with the pathogen. Four isolates showed more efficacies when inoculated previously four hours before the pathogen. The isolate denominated B24 was the only that showed an important percent inhibition (84.04 %) at the lower concentration tested (3x103 CFU/ml) and the isolate RA9 was the less effective strain.\n","PeriodicalId":53282,"journal":{"name":"Anales de Biologia","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Effect of preventive and simultaneous inoculations of Bacillus amyloliquefaciens (Fukumoto) strains on conidial germination of Botrytis cinerea Pers.:Fr.\",\"authors\":\"A. Hamdache, M. Ezziyyani, A. Lamarti\",\"doi\":\"10.6018/ANALESBIO.40.08\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\nBotrytis cinerea es un patógeno fúngico necrotrófico de varias plantas distribuido en todo el mundo. En el presente estudio se analizó el efecto de nueve aislados bacterianos sobre la germinación de conidios de B. cinerea. Los aislados se identificaron como Bacillus amyloliquefaciens. Para evaluar la eficacia de los aislados, se realizaron confrontaciones duales tanto preventivas como simultáneas con los conidios de B. cinerea. Los resultados mostraron que cinco aislados fueron más eficaces cuando se co-inocularon simultáneamente con el patógeno. Cuatro aislados fueron más eficaces cuando se inocularon previamente cuatro horas antes el patógeno. La cepa denominada B24, mostró una inhibición de (84.04%) en concentraciones muy bajas (3x103 UFC/ml) y la cepa RA9 fue la cepa menos eficaz.\\n\\n\\nBotrytis cinerea is a necrotrophic fungal plant pathogen distributed worldwide. In the current study, the effect of nine bacterial isolates, on germination of B. cinerea conidia were studied. The nine isolates were identified as Bacillus amyloliquefaciens. The efficacy of isolates was tested, at different concentrations, in preventive and simultaneous inoculations with B. cinerea conidia. Results showed that five Bacillus isolates were more effective when co-inoculated simultaneously with the pathogen. Four isolates showed more efficacies when inoculated previously four hours before the pathogen. The isolate denominated B24 was the only that showed an important percent inhibition (84.04 %) at the lower concentration tested (3x103 CFU/ml) and the isolate RA9 was the less effective strain.\\n\",\"PeriodicalId\":53282,\"journal\":{\"name\":\"Anales de Biologia\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-06-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anales de Biologia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.6018/ANALESBIO.40.08\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anales de Biologia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.6018/ANALESBIO.40.08","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of preventive and simultaneous inoculations of Bacillus amyloliquefaciens (Fukumoto) strains on conidial germination of Botrytis cinerea Pers.:Fr.
Botrytis cinerea es un patógeno fúngico necrotrófico de varias plantas distribuido en todo el mundo. En el presente estudio se analizó el efecto de nueve aislados bacterianos sobre la germinación de conidios de B. cinerea. Los aislados se identificaron como Bacillus amyloliquefaciens. Para evaluar la eficacia de los aislados, se realizaron confrontaciones duales tanto preventivas como simultáneas con los conidios de B. cinerea. Los resultados mostraron que cinco aislados fueron más eficaces cuando se co-inocularon simultáneamente con el patógeno. Cuatro aislados fueron más eficaces cuando se inocularon previamente cuatro horas antes el patógeno. La cepa denominada B24, mostró una inhibición de (84.04%) en concentraciones muy bajas (3x103 UFC/ml) y la cepa RA9 fue la cepa menos eficaz.
Botrytis cinerea is a necrotrophic fungal plant pathogen distributed worldwide. In the current study, the effect of nine bacterial isolates, on germination of B. cinerea conidia were studied. The nine isolates were identified as Bacillus amyloliquefaciens. The efficacy of isolates was tested, at different concentrations, in preventive and simultaneous inoculations with B. cinerea conidia. Results showed that five Bacillus isolates were more effective when co-inoculated simultaneously with the pathogen. Four isolates showed more efficacies when inoculated previously four hours before the pathogen. The isolate denominated B24 was the only that showed an important percent inhibition (84.04 %) at the lower concentration tested (3x103 CFU/ml) and the isolate RA9 was the less effective strain.