禽类的蛋品质:蛋清蛋白的电泳特性

Meziani Samira, D. El-Mokhtar, Menadi Noureddine, D. Mustapha, Z. Mohammed, Bouterfes Mohammed, Benali Mohammed
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引用次数: 0

摘要

本研究的目的是评估和比较蛋清的理化组成,并表征和估计不同鸟类(鸭、鹌鹑、鸡、鹧鸪和鹅)蛋清蛋白的定量变化。对每种鸟类的全蛋温度、重量、高度、密度和形状指数进行了评估。打碎鸡蛋后,进行目视检查,然后测量卵黄(VI)和白蛋白(AI)指数以及高单位(HU)。采用琼脂凝胶电泳法对蛋清蛋白的质量和性质进行了研究。鹌鹑和鹅的总蛋重变化范围分别为(8.98±0.82g至142.91±10.34g)。在所有品种中,蛋黄的厚度几乎是蛋清的三倍。用HU测定的鸡蛋新鲜度,鹧鸪较高,其次是鹌鹑和鸡,分别为88.81±0.09、87.45±3.33和81.53±2.16。VI显示的值约为0.40。研究的所有当地物种的蛋黄/蛋清比例在47之间,pH值在8.37至8.95之间。所有物种的蛋清蛋白含量均较高(高达14.45g/100g)。通过琼脂糖凝胶电泳鉴定了三种类型的蛋白质(卵清蛋白、卵转铁蛋白和卵清蛋白),不同鸟类之间存在定量差异。密度分析显示,由于蛋清中存在C型溶菌酶,因此存在定性差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Egg Quality from Avian Species: Electrophoretic Characterization of Egg White Proteins
The aim of the present study was to evaluate and to compare the physicochemical composition of egg white, and to characterize and estimate the quantitative variations of egg white proteins of different birds’ species (duck, quail, Chicken, partridge and goose). For each bird species, the whole egg temperature, weight, height, density and shape index were evaluated. After breaking the eggs, a visual examination was performed then vitelline (VI) and albumen (AI) indexes and the haugh unit (HU) were measured. The study of the quality and the characterization of egg white proteins were performed by Agarose Gel Electrophoresis. The total egg weight varied within a range of (8.98±0.82 g to 142.91±10.34 g) for quail and goose, respectively. The thickness of the egg yolk was almost three times higher than that of the egg white in all breeds. The egg freshness, measured by HU, was higher in partridge, followed by quail and chicken breeds with values of 88.81±0.09, 87.45±3.33, 81.53±2.16, respectively. The VI shows values of about 0.40. The egg yolk/ white ratio ranged from 47 and the pH varied from 8.37 to 8.95 for all local species studied. The egg white protein content was higher for all species (up to 14.45g/100 g). Three types of proteins are identified by agarose gel electrophoresis (ovalhibitor, ovotransferrin and ovalbumin) with a quantitative difference between bird species. Densitometric analysis shows a qualitative difference due to the presence of type C lysozyme in chicken egg white.
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