lncRNA-mtb在结核分枝杆菌感染巨噬细胞中的作用

Q4 Immunology and Microbiology

中华微生物学和免疫学杂志 Pub Date : 2019-11-30 DOI:10.3760/CMA.J.ISSN.0254-5101.2019.11.007

Jiang Zichao, Hu Zhidong, Luo Wei, Wang Qian

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引用次数: 0

摘要

目的探讨lncRNA-mtb在结核分枝杆菌感染巨噬细胞中的作用机制。方法采用预测软件和Western blot方法验证lncRNA-mtb的非编码性。RT-qPCR检测不同分枝杆菌感染巨噬细胞中lncRNA-mtb的表达。采用RNA干扰(RNAi)技术沉默巨噬细胞lncRNA-mtb表达后，ELISA检测细胞因子分泌情况。采用Western blot和RT-qPCR分析lncRNA-mtb在结核分枝杆菌感染过程中是否能诱导巨噬细胞炎症活化。研究lncRNA-mtb沉默对结核分枝杆菌感染巨噬细胞杀菌活性的影响。结果lncRNA-mtb在感染结核分枝杆菌H37Rv和H37Ra的巨噬细胞中表达升高。沉默lncRNA-mtb表达后，感染H37Rv的巨噬细胞IL-1β分泌明显减少。进一步研究发现lncRNA-mtb可能参与了结核分枝杆菌感染巨噬细胞NLRP3的激活。沉默lncRNA-mtb表达后，巨噬细胞对H37Ra的杀菌活性受损。结论H37Rv和H37Ra感染巨噬细胞可诱导lncRNA-mtb表达增强。此外，lncRNA-mtb参与了M. tuberculosis诱导的细胞炎症反应。沉默lncRNA-mtb会减弱巨噬细胞清除结核分枝杆菌的能力。关键词:结核分枝杆菌;lncRNA-mtb;巨噬细胞;炎症

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Role of lncRNA-mtb in Mycobacterium tuberculosis-infected macrophages

Objective To study the mechanism of lncRNA-mtb in Mycobacterium tuberculosis (M.tuberculosis)-infected macrophages. Methods A predicting software and Western blot assay were used to verify the non-coding nature of lncRNA-mtb. RT-qPCR was performed to detect lncRNA-mtb expression in macrophages infected by different Mycobacteria. Cytokine secretion was detected with ELISA after silencing the expression of lncRNA-mtb in macrophages with RNA interference (RNAi) technology. Western blot and RT-qPCR were performed to analyze whether the activation of macrophage inflammation could be induced by lncRNA-mtb during M. tuberculosis infection. Effects of lncRNA-mtb silencing on the bactericidal activity of M. tuberculosis-infected macrophages were evaluated. Results Increased expression of lncRNA-mtb was observed in macrophages infected with M. tuberculosis H37Rv or H37Ra. After silencing the expression of lncRNA-mtb, IL-1β secretion in H37Rv infected-macrophages was significantly decreased. Further studies revealed that lncRNA-mtb might be involved in the activation of NLRP3 in M. tuberculosis-infected macrophages. The bactericidal activity of macrophages against H37Ra was impaired after silencing the expression of lncRNA-mtb. Conclusions Enhanced expression of lncRNA-mtb could be induced in macrophages infected with H37Rv or H37Ra. Moreover, lncRNA-mtb was involved in M. tuberculosis-induced inflammation in cells. Silencing lncRNA-mtb wolud attenuate the ability of macrophages to clear M. tuberculosis. Key words: Mycobacterium tuberculosis; lncRNA-mtb; Macrophage; Inflammation

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来源期刊

中华微生物学和免疫学杂志 Immunology and Microbiology-Virology

CiteScore

0.50

自引率

0.00%

发文量

6906

期刊介绍： Chinese Journal of Microbiology and Immunology established in 1981. It is one of the series of journal sponsored by Chinese Medical Association. The aim of this journal is to spread and exchange the scientific achievements and practical experience in order to promote the development of medical microbiology and immunology. Its main contents comprise academic thesis, brief reports, reviews, summaries, news of meetings, book reviews and trends of home and abroad in this field. The distinguishing feature of the journal is to give the priority to the reports on the research of basic theory, and take account of the reports on clinical and practical skills.