用于监测埃及伊蚊洛克菲勒在节肢动物控制设施中饲养的安全性的分子工具

IF 0.7 4区 农林科学 Q4 ENTOMOLOGY
Marina E. Battaglia, L. Díaz-Nieto, C. Berón
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引用次数: 0

摘要

摘要自从人们认识到埃及伊蚊通过这种蚊子传播人类和动物病原体以来,世界上大多数实验室对埃及伊蚊(Diptera:库蚊科)昆虫系的兴趣和使用都有所增加,这导致了对热带疾病和媒介的进一步科学研究,以及开发用于控制蚊子种群的化学和生物产品。近年来,减少蚊子数量的方法集中在新技术上,包括释放沃尔巴克氏体感染株、转基因载体和在无菌昆虫技术中受到辐射的昆虫。为了评估其中的一些技术,必须在实验室条件下准确鉴定和维持野生埃及伊蚊种群和参考菌株。这项工作提出了一种新的工具来监测参考蚊子株与邻近地区的埃及伊蚊野生本地种群之间,或同一昆虫群落中不同品系之间可能的交流。我们比对了来自不同来源的埃及伊蚊ND5基因片段,在Rockefeller(Rock)菌株的个体和不同的野生埃及伊蚊种群之间发现了一个具有假定单核苷酸多态性的区域。分子标记中的这些多态性位点使我们能够将Rock参考菌株与在阿根廷东南部以及与巴西、乌拉圭和巴拉圭接壤的地区发现的野生埃及伊蚊单倍型区分开来,它可以作为一种工具,用于不同节肢动物控制水平的蚊子昆虫群落的调控实体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular tool for monitoring the safety of Aedes (Stegomyia) aegypti Rockefeller rearing in arthropod containment facilities
Abstract The interest in and use of Aedes (Stegomyia) aegypti (Linnaeus) (Diptera: Culicidae) insectary lines increased in most laboratories around the world since the recognition of the transmission of human and animal pathogens by this mosquito species, resulting in further scientific research on tropical diseases and vectors, and the development of chemical and biological products for mosquito populations control. In recent years, approaches to mosquito populations reduction have focused on new technologies that include the release of Wolbachia-infected lines, genetically modified vector and insects subjected to radiation in the Sterile Insect Technique. In order to evaluate some of these techniques, it is essential to count with wild A. aegypti populations and the reference strain, accurately identified, maintained under laboratory conditions. This work proposes a new tool to monitor possible exchanges between reference mosquito strain and wild native populations of A. aegypti in neighboring areas, or between different lines in the same insectary. We aligned and compared ND5 gene fragments of A. aegypti from diverse sources, finding a region with putative Single Nucleotide Polymorphisms between individuals of Rockefeller (Rock) strain and different wild A. aegypti populations. These polymorphic sites in the molecular marker, allowed us to discriminate Rock reference strain from the wild A. aegypti haplotypes found in the southeast of Argentina and bordering areas with Brazil, Uruguay and Paraguay, and it can be useful as a tool for regulatory entities of mosquito insectaries at different Arthropod Containment Levels.
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来源期刊
CiteScore
1.50
自引率
12.50%
发文量
14
审稿时长
6-12 weeks
期刊介绍: Information not localized
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